Abstract
Aim
To determine the efficacy of salivary nitric oxide as a biomarker for dental caries risk in children and to compare the salivary nitric oxide levels in caries-active and caries-free children.
Methods
One hundred children aged 6–12 years were selected and were divided into two groups according to DMFT index: as caries-free consisting of 50 individuals (DMFT/dmft = 0) and caries-active group (DMFT/dmft ≥3) consisting of 50 individuals. The saliva collected was estimated for nitric oxide level by Griess reaction method. Paired t test was used to correlate the difference of mean salivary nitric oxide levels between caries-active and caries-free groups.
Results
The mean level of nitric oxide in saliva of the control group was 581.3 ± 134.6 μg/mL and that of caries-active group was 335.4 ± 111.1 μg/mL, respectively. The mean salivary level of nitric oxide was higher in the caries-free group as compared with the caries-active group which was statistically significant (p = 0.000).
Conclusions
Salivary nitric oxide was higher in the caries-free group as compared to the caries-active group which suggests that increased production of nitric oxide in the oral cavity may contribute to lower caries incidence in children.
Similar content being viewed by others
References
Andrukhov O, Haririan H, Bertl K, et al. Nitric oxide production, systemic inflammation and lipid metabolism in periodontitis patients: possible gender aspect. J Clin Periodontol. 2013;40(10):916–23.
Axelsson S. Effect of combined caries preventive methods: a systematic review of controlled clinical trials. Acta Odontol Scand. 2004;62:163–9.
Bayindir YZ, Polat MF, Seven N. Nitric oxide concentrations in saliva and dental plaque in relation to caries experience and oral hygiene. Caries Res. 2005;39(2):130–3.
Carossa S, Pera P, Doglio P, et al. Oral nitric oxide during plaque deposition. Eur J Clin Investig. 2001;31(10):876–9.
Chow SC, Shao J, Wang H. Sample size calculations in clinical research. Boca Raton: Taylor and Francis; 2003. p. 63–8.
De Soet JJ, Nyvad B, Kilian M. Strain-related acid production by oral streptococci. Caries Res. 2000;34(6):486–90.
Dervisevic A, Babic N, Huskic J, et al. Concentration of nitric oxide in saliva of patients with rheumatoid arthritis. Int J Collab Res Intern Med Public Health. 2012;4(7):1442–51.
Disney JA, Graves RC, Stamm JW, et al. The University of North Carolina caries risk assessment study: further developments in caries risk prediction. Community Dent Oral Epidemiol. 1992;20(2):64–75.
Doel JJ, Hector MP, Amirtham CV, et al. Protective effect of salivary nitrate and microbial nitrate reductase activity against caries. Eur J Oral Sci. 2004;112(5):424–8.
Doel JJ, Benjamin N, Hector MP, Rogers M, Allaker RP. Evaluation of bacterial nitrate reduction in the human oral cavity. Eur J Oral Sci. 2005;113(1):14–9.
Doniger SB (2005) Saliva and oral health. Career and Technical Education, 25:52–55.
Duncan C, Dougall H, Johnson P, et al. Chemical generation of nitric oxide in the mouth from enterosalivary circulation of dietary nitrate. Nat Med. 1995;1(6):546–51.
Duncan C, Li H, Dykhuizen R, et al. Protection against oral and gastrointestinal disease: importance of dietary nitrate intake, oral nitrate reduction and enterosalivary nitrite circulation. Comp Biochem Physiol A Physiol. 1997;118(4):939–48.
Fontana M, Zero DT. Assessing patient’s caries risk. J Am Dent Assoc. 2006;137(9):1231–9.
Geddes DAM. Acids produced by human dental plaque metabolism in situ. Caries Res. 1975;9:98–109.
Gopinath VK, Arzreanne AR. Saliva as a diagnostic tool for assessment of dental caries. Arch Orofac Sci. 2006;1(1):57–9.
Green LC, Wagner DA, Glogowski J, et al. Analysis of nitrate, nitrite and (13N) in biological fluids. Anal Biochem. 1982;126(1):131–8.
Hegde AM, Neekhra V, Shetty S. Evaluation of levels of nitric oxide in saliva of children with rampant caries and early childhood caries: a comparative study. J Clin Pediatr Dent. 2008;32(4):283–6.
Hord NG, Tang Y, Bryan NS. Food sources of nitrates and nitrites: the physiologic context for potential health benefits. Am J Clin Nutr. 2009;90(1):1–10.
Johnson NW. Dental caries: markers of high and low risk groups and individuals. Cambridge: Cambridge University Press; 1991. p. 142–55.
Kathariya R, Pradeep RA. Salivary proteomic biomarkers for oral diseases: a review of literature. Arch Oral Sci Res. 2010;1(1):43–9.
Kojima H, Nakatsubo N, Kikuchi K, et al. Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. Anal Chem. 1998;70(13):2446–53.
Lenander-Lumikari M, Loimaranta V. Saliva and dental caries. Adv Dent Res. 2000;14:40–7.
Lundberg JO, Weitzberg E, Lundberg JM, Alving K. Intragastric nitric oxide production in humans: measurement in expelled air. Gut. 1994;35(11):1543–6.
Mithra NH, Nidarsh DH, Aparna A, Shilpa S. Salivary nitric oxide as biomarker of dental caries in adults: an in vivo study. Int Res J Pharm. 2012;3(11):100–2.
Moncada S, Higgs A. The l-arginine nitric oxide pathway. N Engl J Med. 1993;329(27):2002–12.
Moshage H, Kok B, Huizenga JR, Jansen LM. Nitrite and nitrate determination in plasma: a critical evaluation. Clin Chem. 1995;41:892–6.
Slavkin HC. Protecting the mouth against microbial infections. J Am Dent Assoc. 1998;129:1138–43.
Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors. 2003;3:276–84.
Surdilovic D, Stojanovic I, Apostolovic M, Igic M, Kostadinovic L. The role of nitric oxide in saliva in reduction of caries. Acta Fac Med Naiss. 2008;25(2):93–5.
Vadiakas G. Case definition, aetiology and risk assessment of early childhood caries (ECC): a revisited review. Eur Arch Paediatr Dent. 2008;9(3):114–25.
Vuolteenaho K, Moilanen T, Knowles RG, Moilanen E. The role of nitric oxide in osteoarthritis. Scand J Rheumatol. 2007;36(4):247–58.
Yang F, Troncy E, Francoeur M, et al. Effect of reducing agents and temperatures on conversion of nitrite and nitrate to nitric oxide and detection of nitric oxide by chemiluminescence. Clin Chem. 1997;43(4):657–62.
Zero D, Fontana M, Lennon AM. Clinical applications and outcomes of using indicators of risk in caries management. J Dent Educ. 2001;65(10):1126–32.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
All the procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study obtained ethical approval from the institutional ethical clearance committee of ITS-CDSR.
Informed consent
Informed consent was obtained from all individual participants included in the study.
Funding
This study was not funded by any organisation or institution.
Conflict of interest
All the authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Syed, M., Sachdev, V. & Chopra, R. Intercomparison of salivary nitric oxide as a biomarker of dental caries risk between caries-active and caries-free children. Eur Arch Paediatr Dent 17, 239–243 (2016). https://doi.org/10.1007/s40368-016-0234-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40368-016-0234-z