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Rapid absolute determination platform of nucleic acid for point-of-care testing

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Chemical Research in Chinese Universities Aims and scope

Abstract

Nowadays nucleic acid tests are promising to be considered as point-of-care testing(POCT). However, no such devices are currently available that can perform all the functions, including absolute nucleic acid determination, worldwide on-site detection, rapid analysis and real-time results reporting via ubiquitous mobile networks simultaneously with full package and automated means of measurement. In this study, we presented a compact low-cost portable POC automated testing platform with all attributes mentioned above. A disposable self-priming compartmentalization(SPC) microfluidic chip is used to conduct isothermal amplification. The platform also includes a micro-computer controlled heating unit, an inexpensive optical imaging setup, and a mobile device with customized software. It may become a useful tool for the rapid on-site detection of infectious diseases as well as other pathogens.

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Authors and Affiliations

  1. Research Center for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310058, P. R. China

    Bofan Song, Wei Jin, Qi Song, Qinhan Jin & Ying Mu

Authors
  1. Bofan Song
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  2. Wei Jin
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  3. Qi Song
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  4. Qinhan Jin
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  5. Ying Mu
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Corresponding authors

Correspondence to Wei Jin or Ying Mu.

Additional information

Supported by the National Natural Science Foundation of China (No.31270907), the National Key Foundation for Exploring Scientific Instrument of China(No.2013YQ470781) and the Scientific Research Project of Shanghai City Science and Technology Committee, China(No.14495810400).

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Song, B., Jin, W., Song, Q. et al. Rapid absolute determination platform of nucleic acid for point-of-care testing. Chem. Res. Chin. Univ. 31, 519–525 (2015). https://doi.org/10.1007/s40242-015-4503-x

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  • DOI: https://doi.org/10.1007/s40242-015-4503-x

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