Skip to main content
SpringerLink
Log in

Gene manipulation based selenium-containing peptide exhibiting synergism of SOD and GPx

  • Published:
Chemical Research in Chinese Universities Aims and scope

Abstract

In this paper, we constructed a novel bifunctional superoxide dismutase(SOD)/glutathione peroxidase(GPx) mimic, a selenium-, copper-containing 35-mer peptide conjugate(Se-Cu-35P) in which a three-amino acid linker(Gly-Asn-Gly) connects the C-terminus of 17-mer polypeptide SOD mimic with the N-terminus of 15-mer polypeptide GPx mimic. The SOD and GPx activities of Se-Cu-35P are two orders of magnitude lower than those of natural SOD and GPx, respectively. It provides a GPx activity 56-fold higher than Ebselen(a well-known GPx mimic). The glutathione(GSH) binding constant is 5.6×102 L·mol−1. Se-Cu-35P synergistically resists against the inactivation by H2O2 and protects the mitochondria from oxidative damage in a dose dependent manner. These results highlight the challenge of generating an efficient SOD/GPx synergism mimic. It could facilitate the studies of the cooperation of GPx and SOD and could be a potential therapeutic agent for the treatment of ROS-mediated diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Harman D., Ann. N. Y. Acad. Sci., 2006, 1067, 10

    Article  CAS  Google Scholar 

  2. El Assar M., Angulo J., Rodriguez-Manas L., Free Radical Bio. Med., 2013, 65, 380

    Article  Google Scholar 

  3. Deng F., Zhao Z. W., Arch. Insect. Biochem., 2014, 86, 46

    CAS  Google Scholar 

  4. Benedetti E., D’Angelo B., Cristiano L., Di Giacomo E., Fanelli F., Moreno S., Cecconi F., Fidoamore A., Antonosante A., Falcone R., Ippoliti R., Giordano A., Cimini A., Cell Cycle, 2014, 13, 1335

    Article  CAS  Google Scholar 

  5. Delmastro-Greenwood M. M., Tse H. M., Piganelli J. D., Antioxid. Redox Sign., 2014, 20, 2465

    Article  CAS  Google Scholar 

  6. Blein S., Berndt S., Joshi A. D., Campa D., Ziegler R. G., Riboli E., Cox D. G., Free Radical Res., 2014, 48, 380

    Article  CAS  Google Scholar 

  7. Loft S., Danielsen P. H., Mikkelsen L., Risom L., Forchhammer L., Moller P., Biochem. Soc. T., 2008, 36, 1071

    Article  CAS  Google Scholar 

  8. Hodgson E. K., Fridovich I., Biochemistry, 1975, 14, 5294

    Article  CAS  Google Scholar 

  9. Shen N., Yan F., Guo Y., Lue S. W., Gong P. S., Xu Y. W., Yan G. L., Mu Y., Luo G. M., Chem. Res. Chinese Universities, 2011, 27(2), 258

    CAS  Google Scholar 

  10. Yan F., Mu Y., Yan G. L., Liu J. Q., Shen J. C., Luo G. M., Mini-Rev. Med. Chem., 2010, 10, 342

    Article  CAS  Google Scholar 

  11. Baudry M., Etienne S., Bruce A., Palucki M., Jacobsen E., Malfroy B., Biochem. Bioph. Res. Co., 1993, 192, 964

    Article  CAS  Google Scholar 

  12. Dong Z. Y., Huang X., Mao S. Z., Liu J. Q., Luo G. M., Shen J. C., Chem. Lett., 2005, 34, 820

    Article  CAS  Google Scholar 

  13. Li H. M., Gao N., Liu L., Shen S. L., Luo G. M., Chem. J. Chinese Universities, 2009, 30(7), 1353

    Google Scholar 

  14. Xu Y., Liu B., Zweier J. L., He G. L., J. Pharmacol. Exp. Ther., 2008, 327, 402

    Article  CAS  Google Scholar 

  15. Umakoshi H., Morimoto K., Ohama Y., Nagami H., Shimanouchi T., Kuboi R., Langmuir, 2008, 24, 4451

    Article  CAS  Google Scholar 

  16. Lanza V., Vecchio G., J. Inorg. Biochem., 2009, 103, 381

    Article  CAS  Google Scholar 

  17. Liu Y., Li B., Li L., Zhang H. Y., Helv. Chim. Acta, 2002, 85, 9

    Article  CAS  Google Scholar 

  18. Yu H. J., Ge Y., Wang Y., Lin C. T., Li J., Liu X. M., Zang T. Z., Xu J. Y., Liu J. Q., Luo G. M., Shen J. C., Biochem. Bioph. Res. Co., 2007, 358, 873

    Article  CAS  Google Scholar 

  19. Yan F., Yang W. K., Li X. Y., Lin T. T., Lun Y. N., Lin F., Lv S. W., Yan G. L., Liu J. Q., Shen J. C., Mu Y., Luo G. M., BBA-Gen. Subjects, 2008, 1780, 869

    Article  CAS  Google Scholar 

  20. Gao Y. Z., Hou C. X., Zhou L. P., Zhang D. M., Zhang C. Q., Miao L., Wang L., Dong Z. Y., Luo Q., Liu J. Q., Macromol. Biosci., 2013, 13, 808

    Article  CAS  Google Scholar 

  21. Zou X. F., Ji Y. T., Gao G., Zhu X. J., Lv S. W., Yan F., Han S. P., Chen X., Gao C. C., Liu J. Q., Luo G. M., J. Microbiol. Biotechn., 2010, 20, 88

    CAS  Google Scholar 

  22. Yan F., Yan G. L., Lv S. W., Shen N., Mu Y., Chen T., Gong P. S., Xu Y. W., Lv L. M., Liu J. Q., Shen J. C., Luo G. M., Int. J. Biochem. Cell B., 2011, 43, 1802

    Article  CAS  Google Scholar 

  23. Luo G. M., Chen H. P., Cheng Y. H., Ann. N.Y. Acad. Sci., 1988, 542, 79

    Article  CAS  Google Scholar 

  24. Sun Y., Li T. Y., Chen H., Zhang K., Zheng K. Y., Mu Y., Yan G. L., Li W., Shen J. C., Luo G. M., J. Biol. Chem., 2004, 279, 37235

    Article  CAS  Google Scholar 

  25. Wilson D. R., Finlay B. B., Protein Eng., 1997, 10, 519

    Article  CAS  Google Scholar 

  26. Cho M. K., Lee C. J., Lee C. H., Li S. Z., Lim S. K., Baik J. H., Lee W., J. Struct. Biol., 2005, 150, 300

    Article  CAS  Google Scholar 

  27. Epp O., Ladenstein R., Wendel A., Eur. J. Biochem., 1983, 133, 51

    Article  CAS  Google Scholar 

  28. Strub M. P., Hoh F., Sanchez J. F., Strub J. M., Bock A., Aumelas A., Dumas C., Structure, 2003, 11, 1359

    Article  CAS  Google Scholar 

  29. Rae T. D., Torres A. S., Pufahl R. A., O’Halloran T. V., J. Biol. Chem., 2001, 276, 5166

    Article  CAS  Google Scholar 

  30. Schagger H., Nat. Protoc., 2006, 1, 16

    Article  Google Scholar 

  31. Lu B. C., Dong S., Xue Q., Zong H., Lu S. W., Luo G. M., Chem. J. Chinese Universities, 2013, 34(9), 2146

    Google Scholar 

  32. Zhang K., Zang T. Z., Yang W., Sun Y., Mu Y., Liu J. Q., Shen J. C., Luo G. M., J. Biol. Chem., 2006, 281, 12516

    Article  CAS  Google Scholar 

  33. Lansman R. A., Shade R. O., Shapira J. F., Avise J. C., J. Mol. Evol., 1981, 17, 214

    Article  CAS  Google Scholar 

  34. Hunter F. E. Jr., Scott A., Hoffsten P. E., Guerra F., Weinstein J., Schneider A., Schutz B., Fink J., Ford L., Smith E., J. Biol. Chem., 1964, 239, 604

    CAS  Google Scholar 

  35. Pryor W. A., Stanley J. P., Blair E., Lipids, 1976, 11, 370

    Article  CAS  Google Scholar 

  36. Stamova S., Cartellieri M., Feldmann A., Arndt C., Koristka S., Bartsch H., Bippes C. C., Wehner R., Schmitz M., von Bonin M., Bornhauser M., Ehninger G., Bachmann M., Mol. Immunol., 2011, 49, 474

    Article  CAS  Google Scholar 

  37. Westerhof L. B., Wilbers R. H., Roosien J., van de Velde J., Goverse A., Bakker J., Schots A., PLoS One, 2012, 7, e46460

    Article  CAS  Google Scholar 

  38. Sun W., Hu Y. J., Gong J. W., Zhu C. B., Zhu B. Q., Biochemistry-Moscow, 2005, 70, 753

    Article  CAS  Google Scholar 

  39. Gong Q., Yang X., Cai W., Gao G., Yang Z., Protein J., 2010, 29, 167

    Article  CAS  Google Scholar 

  40. Mugesh G., Singh H. B., Chem. Soc. Rev., 2000, 29, 347

    Article  CAS  Google Scholar 

  41. Marklund S. L., Proc. Natl. Acad. Sci. USA, 1982, 79, 7634

    Article  CAS  Google Scholar 

  42. Jewett S. L., Rocklin A. M., Ghanevati M., Abel J. M., Marach J. A., Free Radic. Biol. Med., 1999, 26, 905

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, 130021, P. R. China

    Na Shen, Fei Yan, Shaowu Lü, Wenkui Yang, Guimin Luo, Ganglin Yan & Ying Mu

  2. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310058, P. R. China

    Ying Mu

Authors
  1. Na Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fei Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shaowu Lü
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wenkui Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guimin Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ganglin Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ying Mu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ganglin Yan or Ying Mu.

Additional information

Supported by the National Natural Science Foundation of China(Nos.31170740, 31270907) and the Natural Science Foundation of Jilin Province, China(No.20130101159JC).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shen, N., Yan, F., Lü, S. et al. Gene manipulation based selenium-containing peptide exhibiting synergism of SOD and GPx. Chem. Res. Chin. Univ. 30, 947–952 (2014). https://doi.org/10.1007/s40242-014-4239-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40242-014-4239-z

Keywords

Search

Navigation