Date: 24 Sep 2013

Early Diagnosis of Sex in Jojoba, Simmondsia chinensis (Link) Schneider by Sequence Characterized Amplified Region Marker

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Abstract

Simmondsia chinensis (Link) Schneider is a dioecious shrub in which female plants are economically more important than the male plants, as seeds produced by the female plants store liquid wax ester that is extensively used in cosmetic industry. Sex of jojoba individuals can be determined only at flowering stage which comes after 3–4 years of seeding. Since, population of jojoba is male biased, so, to identify female plants at seedling stage, more reliable and robust male-specific sequence characterized amplified region marker was developed from male-specific inter-simple sequence repeat marker. During homology searching, no DNA sequence with significant similarity to male-specific inter-simple sequence repeat marker was found in non-redundant database of National Center for Biotechnology Information. Sequence characterized amplified region primer pair designed, based on the sequence of inter-simple sequence repeat marker, (GenBank accession no. HQ166029.1) amplified a fragment approx. 1000 bp in male plants only. This fragment was completely absent in female plants. Authentication of marker was done by using samples taken from two provinces of India.