Abstract
The present work was aimed at the determination of distribution of lactic acid bacteria on aerial surfaces of pomegranate, and evaluation of the antifungal potential for their possible use as biocontrol agent. Maximum population of lactic acid bacteria was detected on flowers of Bhagwa and Ganesh variety, although the count was higher in Bhagwa (P < 0.05). The population of lactic acid bacteria on different sample types was significant (P < 0.05) during April and October season, and the count was always higher in October season. Potent antifungal isolates were identified by phenotypic characters (Gram positive card kit) and partial 16S rDNA sequencing as Lactobacillus brevis, Enterococcus faecium, Lactococcus lactis ssp. cremoris and Lactococcus lactis ssp. lactis. Of the 200 lactic acid bacteria isolates, 124 were antagonistic towards Fusarium graminearum as determined by agar overlay technique. Out of these, 26 were able to retain the antifungal activity after long term storage of 12 months at 4 °C. Thus, pomegranate plant is an excellent source for diverse antifungal lactic acid bacteria.
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Gajbhiye, M., Prakash, D., Jagdale, S. et al. Pomegranate Borne Fungicidal Lactic Acid Bacteria and their Biodiversity. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 82, 413–419 (2012). https://doi.org/10.1007/s40011-012-0055-8
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DOI: https://doi.org/10.1007/s40011-012-0055-8