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The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples

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Abstract

Detection limits of PCR to detect Cryptosporidium spp. were determined in ninety-six preserved fecal samples (obtained for a different project). Small subunit ribosomal RNA gene was amplified from each sample. The best detection limit of the PCR method was > 150 oocysts.

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Authors and Affiliations

  1. Biology Department, College of Science, University of Baghdad, Al-Jadriyah Campus, Baghdad, Iraq

    Harith Saeed Al-Warid & Souhaila H. Mahmood

  2. School of Natural Resources, University of Missouri, 302 Anheuser-Busch Natural Resources Building, Columbia, MO, 65211, USA

    Harith Saeed Al-Warid

  3. Al-Israa College University, Baghdad, Iraq

    Ihsan M. Al-Saqur

Authors
  1. Harith Saeed Al-Warid
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  2. Ihsan M. Al-Saqur
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  3. Souhaila H. Mahmood
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Correspondence to Harith Saeed Al-Warid.

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Al-Warid, H.S., Al-Saqur, I.M. & Mahmood, S.H. The Detection Limit of PCR Amplification for Cryptosporidium spp. Oocysts in Fecal Samples. Natl. Acad. Sci. Lett. 42, 423–424 (2019). https://doi.org/10.1007/s40009-018-0770-7

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  • DOI: https://doi.org/10.1007/s40009-018-0770-7

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