Abstract
Fish in a tropical country like India are frequently exposed to different duration of hypoxia. The effect of hypoxia on the physiology of fish, air-breathing catfish Clarias batrachus were exposed to different duration of hypoxia and its effect on activities of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) were studied in four tissues (heart, liver, brain and muscle). The specific activity of LDH increases in all tissues, which reflects towards onset of anaerobic respiration and decrease in energy demand in all these tissues. In contrast, MDH specific activities were decreased significantly in heart, suggesting involvement of strong aerobic respiration in heart during hypoxia. The present investigation revealed that during hypoxia enzyme activities responded in a tissue-specific manner in the fish C. batrachus reflecting the balance of energetic demands, metabolic role and oxygen supply of particular tissues.
References
Damotharan P, Perumal NV, Arumugam M, Vijaylakshmi S, Balasubramanian T (2010) Seasonal variation of physicochemical characteristics in Point Calimere coastal water south east coast of India. Middle East J Sci Res 64:333–339
Timmerman CM, Chapman LJ (2004) Behavioural and physiological compensation for chronic hypoxia in the Sailfin Molly (Poecilia latipinna). Physiol Biochem Zool 77:601–610
Boutilier RG (2001) Mechanisms of cell survival in hypoxia and hypothermia. J Exp Biol 204:3171–3181
Lutz PL, Nilsson GE (1997) Contrasting strategies for anoxic brain survival—glycolysis up or down. J Exp Biol 200:411–419
Hochachka PW, Somero GN (2002) Biochemical adaptation: mechanism and process in physiological evolution. Oxford University Press, Oxford, p 466
Wilson TL (1977) Interrelations between pH and temperature for the catalytic rate of the M4 isozyme of lactate dehydrogenase (EC 1.1.1.27) from goldfish (Carassius auratus). Arch Biochem Biophys 179:378–390
Graves JE, Somero GN (1982) Electrophoretic and functional enzymic evolution in four species of Eastern Pacific barracudas from different thermal environments. Evolution 36:97–106
Panepucci L, Schwantes ML, Schwantes AR (1987) Biochemical and physiological properties of the lactate dehydrogenase allozymes of the Brazilian Teleost, Leporinus friderici, Anostomidae, Cypriniformes. Comp Biochem Physiol 87B:199–206
Coppes ZL, Somero GN (1990) Temperature differences between the M4 lactate dehydrogenase of stenothermal and eurythermal Sciaenid fishes. J Exp Zool 254:127–131
Almeida-Val VMF, Hochachka PW (1993) Hypoxia tolerance in Amazon: status of an under-explored biological “goldmine”. In: Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) Surviving hypoxia: mechanism of control and adaptation. CRC Press, Boca Raton, pp 435–445
Martinez ML, Christie L, Boehm R, Manning S, Rees BB (2006) Effects of long- term hypoxia on enzymes of carbohydrate metabolism in the Gulf killifish, Fundulus grandis. J Exp Biol 209:3851–3861
Shaklee JB, Christiansen JA, Sidell BD, Prosser CL (1977) Whitt GS Molecular aspects of temperature acclimation in fish: contributions of changes in enzyme activities and isozyme patterns to metabolic reorganization in the green sunfish. J Exp Zool 201:1–20
Schwantes MLB, Schwantes AR (1982) Adaptative features of ectothermic enzymes-I. Temperature effects on the malate dehydrogenase from a temperate fish Leiostomus xanthurus. Comp Biochem Physiol 72B:49–58
Schwantes MLB, Schwantes AR (1982) Adaptative features of ectothermic enzymes-II. Temperature effects on the malate dehydrogenase from a temperate fish Leiostomus xanthurus. Comp Biochem Physiol 72B:49–58
Farias IP, Almeida-Val VMF (1992) Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features. Comp Biochem Physiol 103B:939–943
Almeida-Val VMF, Val AL, Duncan WP, Souza FCA, Paula-Silva MN, Land S (2000) Scaling effects on hypoxia tolerance in the Amazon fish Astronotus ocellatus (Perciformes: Cichlidae): contribution of tissue enzyme levels. Comp Biochem Physiol 125B:219–226
Somero GN, Childress JJ (1980) A violation of the metabolism-size scaling paradigm: activities of glycolytic enzymes in muscle increase in larger-size fish. Physiol Zool 53:322–337
Horecker BL, Kornberg A (1948) The extinction coefficients of the reduced band of pyridine nucleotides. J Biol Chem 175:385–390
Pelletier D, Guderley H, Dutil J-D (1993) Effects of growth rate, temperature, season, and body size on glycolytic enzymes activities in the white muscle of Atlantic cod (Gadus morhua). J Exp Zool 265:477–487
Panepucci L, Fernandes MN, Sanches JR, Rantin FT (2000) Changes in lactate dehydrogenase and malate dehydrogenase activities during hypoxia and after temperature acclimation in the armored fish, Rhinelepis strigosa (Siluriformes, Loricariidae). Rev Brasil Biol 60(2):353–360
Tripathi RK, Mohindra V, Singh A, Kumar R, Mishra RM, Jena JK (2013) Physiological responses to acute experimental hypoxia in the air-breathing Indian catfish, Clarias batrachus (Linnaeus, 1758). J Biosci 38(2):373–383
Acknowledgment
Authors express their gratefulness to the Head, Department of Zoology, University of Allahabad, for providing all the facilities needed. The award of BSR fellowship to one of the authors under RFSMS of U.G.C. SAP-FIST programme is also thankfully acknowledged.
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Kumar, A., Gopesh, A. Effect of Hypoxia and Energy Conservation Strategies in the Air-Breathing Indian Catfish, Clarias batrachus . Natl. Acad. Sci. Lett. 38, 135–137 (2015). https://doi.org/10.1007/s40009-014-0332-6
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DOI: https://doi.org/10.1007/s40009-014-0332-6