Skip to main content
SpringerLink
Log in

Microbiological and Molecular Investigation of Clinically Suspected Caseous Lymphadenitis Cases in Goats

  • Full-Length Research Article
  • Published:
Agricultural Research Aims and scope Submit manuscript

Abstract

Chronic suppurative lymphadenitis characterized by abscess formation is of economic significance in sheep and goats. It is principally caused by Corynebacterium pseudotuberculosis (caseous lymphadenitis, CLA) and Staphylococcus aureus subsp. anaerobius. Pus samples from superficial lymph nodes of 33 (4.38 %) out of 752 adult Sirohi goats showing clinical lesions similar to CLA were collected for laboratory investigations. Sixteen (48.48 %) bacterial isolates from pus were identified as C. pseudotuberculosis, 12 (36.36 %) as catalase-negative S. aureus subsp. anaerobius while samples from 5 cases were negative. The polymerase chain reaction (PCR) assays targeting putative oligopeptide/dipeptide ABC transporter, nicotinamide adenine dinucleotide phosphate oxidoreductase coenzyme F420-dependent and proline iminopeptidase genes of C. pseudotuberculosis when carried out directly on the DNA extracted from pus were positive in the same 16 goats. All the C. pseudotuberculosis isolates were also found to be positive for these genes in the PCR. Isolates of S. aureus subsp. anaerobius were found to be positive for 16S rRNA and nuclease (nuc) gene PCR. The present study shows the incidence of CLA as 4.38, 2.12 and 2.12 % based on the clinical, bacterial culture and direct PCR assay on pus samples, respectively. The incidence of abscess disease due to S. aureus subsp. anaerobius was 1.59 %. The three gene PCR assay developed in the study was found to be specific and rapid than the bacterial culture in detecting bacteria directly in the pus samples and can be applied for the diagnosis and control strategy of CLA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Al-Gaabary MH, El-Sheikh WMA (2002) Epidemiological clinical and preventive studies on caseous lymphadenitis in sheep and goats at Gharbia governarate. In: 10th science congress Faculty of Veterinary Medicine, Assiut University, Egypt, pp 402–417

  2. Al-Gaabary MH, Osman SA, Oreiby AF (2009) Caseous lymphadenitis in sheep and goats clinical epidemiological and preventive studies. Small Rumin Res 87:116–121

    Article  Google Scholar 

  3. Al-Gaabary MH, Osman SA, Ahmed MS, Oreiby AF (2010) Abattoir survey on caseous lymphadenitis in sheep and goats in Tanta, Egypt. Small Rumin Res 94:117–124

    Article  Google Scholar 

  4. Al-Harbi KB (2011) Bacterial isolates from visceral abscesses of sheep at Qassim Saudi Arabia. Afr J Microbiol Res 5:5622–5627

    CAS  Google Scholar 

  5. Al-Harbi KB (2011) Prevalence and etiology of abscess disease in sheep and goats at Qassim region, Saudi Arabia. Vet World 4:495–499

    Article  Google Scholar 

  6. Baird GJ, Fontaine MC (2007) Corynebacterium pseudotuberculosis and its role in ovine caseous lymphadenitis. J Comp Pathol 37:179–210

    Article  Google Scholar 

  7. Brakstad OG, Aasbakk K, Maeland JA (1992) Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol 30:1654–1660

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Cetinkaya B, Karahan M, Atil E, Kalin R, De Baere T, Vaneechoutte M (2002) Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Vet Microbiol 88:75–83

    Article  CAS  PubMed  Google Scholar 

  9. D’Afonseca V, Prosdocimi F, Dorella FA, Pacheco LGC, Moraes PM, Pena I, Ortega JM, Teixeira S, Oliveira SC, Coser EM, Oliveira LM, Oliveira GC, Meyer R, Miyoshi A, Azevedo V (2010) Survey of genome organization and gene content of Corynebacterium pseudotuberculosis. Microbiol Res 165:312–320

    Article  CAS  PubMed  Google Scholar 

  10. De la Fuente R, Suarez G, Schleifer KH (1985) Staphylococcus aureus subsp. nov., the causal agent of abscess disease of sheep. Int J Syst Bacteriol 35:99–102

    Article  Google Scholar 

  11. Dercksen DP, Brinkhof JMA, Dekker-Nooren T, Van Maanen K, Bode CF, Baird G, Kamp EM (2000) A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats. Vet Microbiol 75:167–175

    Article  CAS  PubMed  Google Scholar 

  12. Fontaine MC, Baird GJ (2008) Caseous lymphadenitis. Small Rumin Res 76:42–48

    Article  Google Scholar 

  13. Guimaraes AS, Carmo FB, Pauletti RB, Seyffert N, Ribeiro D, Lage AP, Heinemann MB, Miyoshi A, Azevedo V, Gouveia AMG (2011) Caseous lymphadenitis epidemiology diagnosis and control. IIOAB J 2:33–43

    Google Scholar 

  14. Hommez J, Deveriese LA, Vaneechouutte M, Riegel P, Butaye P, Hassebrouck F (1999) Identification of nonlipophiolic corynebacteria isolated from dairy cow with mastitis. J Clin Microbiol 37:954–957

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Khamis A, Raoult D, La Scola B (2004) rpoB gene sequencing for identification of Corynebacterium species. J Clin Microbiol 42:3925–3931

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Kumar J, Singh F, Tripathi BN, Kumar R, Dixit SK, Sonawane GG (2012) Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan India. Trop Anim Health Prod 44:1319–1322

    Article  PubMed  Google Scholar 

  17. Manning EJB, Cushing HF, Hietala S, Wolf CB (2007) Impact of Corynebacterium pseudotuberculosis infection of serologic surveillance for Johne’s disease in goats. J Vet Diagn Invest 19:187–190

    Article  PubMed  Google Scholar 

  18. Mohan P, Vathsala M, Jayaprakasan V (2008) Comparative characterization of Corynebacterium pseudotuberculosis from goats in Kerala, India and reference strain. Small Rumin Res 74:226–230

    Article  Google Scholar 

  19. Moussa I, Shibl AM (2009) Molecular characterization of methicillin-resistant Staphylococcus aureus recovered from outpatient clinics in Riyadh, Saudi Arabia. Saudi Med J 30:611–617

    PubMed  Google Scholar 

  20. Mubarak M, Bastawrows AF, Abdel-Hafeez MM, Ali MM (1999) Caseous lymphadenitis of sheep and goats in Assiut farms and abattoirs. Assiut Vet Med J 42:89–112

    Google Scholar 

  21. Pacheco LGC, Pena RR, Castro TLP, Dorella FA, Bahia RC, Carminati R, Frota MNL, Oliveira SC, Meyer R, Alves FSF, Miyoshi A, Azevedo V (2007) Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. J Med Microbiol 56:480–486

    Article  CAS  PubMed  Google Scholar 

  22. Paton MW, Waker SB, Rose IR, Watt GF (2003) Prevalence of caseous lymphadenitis and usage of caseous lymphadenitis vaccine in sheep flocks. Aust Vet J 81:91–95

    Article  CAS  PubMed  Google Scholar 

  23. Pavan ME, Robles C, Cairo FM, Marcellino R, Pettinari MJ (2012) Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using RNA polymerase β-subunit gene (rpo B). Res Vet Sci 92:202–206

    Article  CAS  PubMed  Google Scholar 

  24. Quinn PJ, Markey BK, Leonard FC, Hartigan P, Fanning S, FitzPatrick ES (2011) Veterinary microbiology and microbial disease, 2nd edn. Wiley-Blackwell, West Sussex

    Google Scholar 

  25. Sambrook J, Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, New York

  26. Seyffert N, Guimarães AS, Pacheco LG, Portela RW, Bastos BL, Dorella FA, Heinemann MB, Lage AP, Gouveia AMG, Meyer R, Miyoshi A, Azevedo V (2010) High seroprevalence of caseous lymphadenitis in Brazilian goat herds revealed by Corynebacterium pseudotuberculosis secreted proteins-based ELISA. Res Vet Sci 88:50–55

    Article  CAS  PubMed  Google Scholar 

  27. Ter Laak EA, Bosch J, Bijl GC, Schreuder BEC (1992) Double-antibody sandwich enzyme-linked immunosorbent assay and immunoblot analysis used for control of caseous lymphadenitis in goats and sheep. Am J Vet Res 53:1125–1132

    PubMed  Google Scholar 

  28. Williamson LH (2001) Caseous lymphadenitis in small ruminants. Vet Clin N Am Food Anim Pract 17:359–371

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the Director and In-charge, Livestock Farm Section of the institute for providing facilities and support to collect clinical samples. The work has been conducted in the Disease Investigation Project and the Network Project on Veterinary Type Culture Centre (ICAR-NRCE, Hisar).

Author information

Authors and Affiliations

  1. Division of Animal Health, Central Sheep and Wool Research Institute, Avikanagar (Via Jaipur), Rajasthan, India

    B. N. Tripathi, Jyoti Kumar, G. G. Sonawane, Rajiv Kumar & S. K. Dixit

Authors
  1. B. N. Tripathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jyoti Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. G. Sonawane
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rajiv Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. K. Dixit
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. N. Tripathi.

Ethics declarations

Conflict of interest

Authors do not have any conflict of interest for publication of this article.

Ethical Approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tripathi, B.N., Kumar, J., Sonawane, G.G. et al. Microbiological and Molecular Investigation of Clinically Suspected Caseous Lymphadenitis Cases in Goats. Agric Res 5, 413–419 (2016). https://doi.org/10.1007/s40003-016-0233-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40003-016-0233-7

Keywords

Search

Navigation