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Comparative Analysis of Free Amino Acids and Nitrogen in Cotton Leaves Treated with Different Classes’ Insecticides

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Abstract

Application of some insecticides in cotton protection is often associated with secondary biotic stress, for example, huge colonization of aphids. One of the possible reasons for this phenomenon is increased level of nutritious compounds in treated plants. Since amino acids (AA) are necessary for the development of aphids, we studied the levels of free amino acids (FAA) and total nitrogen in cotton plant leaves in response to application of three different class insecticides. The plants were treated with Carbophos, Lannate, and Sumi-alfa in early blooming stage and the leaf samples were taken on the 10th and 13th days of the treatment. Carbophos suppressed the portion of total nitrogen in dry mass by 6 % while sumi-alfa elevated by 16 %. The most abundant AA found in leaf samples were alanine, arginine, glutamine, and cysteine. The highest changes in response to insecticides application were observed for tyrosine, methionine, and arginine (P ≤ 0.05). Compared to control, the greatest differences in total FAA and essential amino acids (EAA) were found on the 13th day of the treatment with Lannate; that 1.76 (P ≤ 0.05) and 1.83 (P ≤ 0.05) times more total and EAA were gathered. Sumi-alfa was found to exert mildest effect on cotton by means of the number of AA affected. However, on the 10th day sumi-alfa caused the highest amount of EAA that 1.67 (P ≤ 0.05) times more, than control, EAA accumulated. Due to altered nutritional value, effects of the tested agrochemicals on cotton should be considered in pesticide management.

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Acknowledgement

We express our sincere thanks to Dr. Shamil Khodjayev and PhD. Navruz Sattarov from Agrotoxicology Laboratory of The Institute for Plant Protection, Ministry of Agriculture and Water Resources of Uzbekistan, for their help to perform insecticide application on cotton plants.

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Authors and Affiliations

  1. A. S. Sadykov Institute of Bioorganic Chemistry of Academy of Sciences of Uzbekistan, 83, M. Ulughbek Str., Tashkent, Uzbekistan

    Akmal Asrorov, Uchkun Ishimov, Jamolitdin Ziyavitdinov, Olga Veshkurova & Shavkat Salikhov

  2. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, 2, Akademicka, Nitra, Slovakia

    Ildiko Matusikova

Authors
  1. Akmal Asrorov
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  2. Ildiko Matusikova
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  3. Uchkun Ishimov
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  4. Jamolitdin Ziyavitdinov
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  5. Olga Veshkurova
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  6. Shavkat Salikhov
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Correspondence to Akmal Asrorov.

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Asrorov, A., Matusikova, I., Ishimov, U. et al. Comparative Analysis of Free Amino Acids and Nitrogen in Cotton Leaves Treated with Different Classes’ Insecticides. Agric Res 4, 277–282 (2015). https://doi.org/10.1007/s40003-015-0172-8

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  • DOI: https://doi.org/10.1007/s40003-015-0172-8

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