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Effect of Glomus mosseae and Piriformospora indica on Growth and Antioxidant Defense Responses of Wheat Plants Under Drought Stress

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Abstract

This experiment aimed to investigate the effects of arbuscular mycorrhizal (Glomus mosseae) and endophytic (Piriformospora indica) fungi on lipid peroxidation, antioxidant enzymes activity, and growth of wheat (Triticum aestivum cv. Azar2) under drought stress conditions. The plants were cultivated in a sterile mixture of soil and sand in a greenhouse. Four levels of fungi inoculations (control, G. mosseae, P. indica, and co-inoculation with both fungi) and three levels of soil moisture conditions (field capacity or FC, −5 and −10 bar) were the treatments. The results showed that with increasing in drought severity, the content of hydrogen peroxide and the activities of catalase [CAT, EC 1.11.1.6], ascorbate peroxidase [APX, EC 1.11.1.11], and peroxidase [POX, EC 1.11.1.7] enzymes increased, while leaf chlorophyll content, plant vegetative growth, and fungi colonization decreased. However, there was no change in lipid peroxidation. The inoculated plants with G. mosseae and P. indica (either alone or mixed) had significantly lower levels of both hydrogen peroxide and lipid peroxidation rate, whereas increased antioxidant enzymes such as CAT, APX and POD, and leaf chlorophyll content. The inoculated wheat plants in various moisture levels had better vegetative growth than the non-inoculated plants. Co-inoculated plants, however, had more root colonization than G. mosseae and P. indica inoculated at all moisture levels. The results of the present study indicate that the plant-fungi symbiosis especially co-inoculation of G. mosseae and P. indica markedly improved the defense mechanisms, drought resistance, and growth of wheat plants.

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Acknowledgments

The authors are grateful to the Maragheh University and Dryland Agricultural Research Institute (DARI) for supporting this study.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Ramin Agricultural and Natural Resources University, Khouzestan, Iran

    Yasser Yaghoubian

  2. Department of Plant Pathology, School of Agriculture, Tarbiat Modares University, P. O. Box: 14115-336, Tehran, Iran

    Ebrahim Mohammadi Goltapeh

  3. Department of Agronomy, Genetic and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

    Hemmatollah Pirdashti

  4. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran

    Ezatollah Esfandiari

  5. Dryland Agricultural Research Institute (DARI), Maragheh, Iran

    Vali Feiziasl

  6. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Sector 125, Noida, 201303, UP, India

    Ajit Varma

  7. Department of Chemical Engineering, University Technology Malaysia, Johor Baharu, Malaysia

    Mimi Haryani Hassim

  8. Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Hossein Kari Dolatabadi

Authors
  1. Yasser Yaghoubian
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  2. Ebrahim Mohammadi Goltapeh
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  3. Hemmatollah Pirdashti
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  4. Ezatollah Esfandiari
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  5. Vali Feiziasl
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  6. Hossein Kari Dolatabadi
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  7. Ajit Varma
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  8. Mimi Haryani Hassim
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Correspondence to Hemmatollah Pirdashti.

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Yaghoubian, Y., Goltapeh, E.M., Pirdashti, H. et al. Effect of Glomus mosseae and Piriformospora indica on Growth and Antioxidant Defense Responses of Wheat Plants Under Drought Stress. Agric Res 3, 239–245 (2014). https://doi.org/10.1007/s40003-014-0114-x

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  • DOI: https://doi.org/10.1007/s40003-014-0114-x

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