Case Report

Infection

, Volume 40, Issue 6, pp 689-694

Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever

  • D. LimontaAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
  • , V. FalcónAffiliated withElectron Microscopy Laboratory, Center for Genetic Engineering and Biotechnology (CIGB)
  • , G. TorresAffiliated withPathology Department, Pedro Kourí Tropical Medicine Institute (IPK)
  • , V. CapóAffiliated withPathology Department, Pedro Kourí Tropical Medicine Institute (IPK)
  • , I. MenéndezAffiliated withElectron Microscopy Laboratory, Center for Genetic Engineering and Biotechnology (CIGB)
  • , D. RosarioAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
  • , Y. CastellanosAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
  • , M. AlvarezAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
  • , R. Rodríguez-RocheAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
    • , M. C. de la RosaAffiliated withElectron Microscopy Laboratory, Center for Genetic Engineering and Biotechnology (CIGB)
    • , A. PavónAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
    • , L. LópezAffiliated withPathology Department, Pedro Kourí Tropical Medicine Institute (IPK)
    • , K. GonzálezAffiliated withPathology Department, Salvador Allende General Hospital
    • , G. GuillénAffiliated withBiomedical Research Division, Center for Genetic Engineering and Biotechnology (CIGB)
    • , J. DiazAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK)
    • , M. G. GuzmánAffiliated withArbovirus Laboratory, Virology Department, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute (IPK) Email author 

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Abstract

Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.

Keywords

Dengue hemorrhagic fever Fatal Tissues Electron microscopy RT-PCR