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Comparison of different concentrations of tetracycline-loaded silk fibroin membranes on the guided bone regeneration in the rat calvarial defect model

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

The objective of this study was to evaluate bone regeneration ability using different concentrations of tetracycline (TC) loaded into silk fibroin membranes (SFMs). Prior to animal experiments, MTT assays and alkaline phosphatase (ALP) assays were performed to evaluate the cellular response of each membrane. Critical sized bone defects (8-mm diameter) on rat calvaria were prepared and covered with SFM containing different concentrations of TC:1% TC (TC1), 5% TC (TC5), 10% TC (TC10), and 0% TC (SFM only). The bone regeneration was evaluated by micro-computerized tomography (μ-CT) and histomorphometric analysis at 4 weeks postoperatively. ALP activity was increased in a dose-dependent manner as the applied TC concentrations. By μ-CT analysis, newly formed bone volume in the TC5 group was significantly higher than that in the SFM only group (P<0.001), the TC1 group (P=0.004), and the TC10 group (P=0.012). From histomorphometric analysis, new bone formation was greater in the TC5 group than in the SFM only group (P=0.003) and the TC1 group (P=0.010). There was no significant difference between the TC5 and TC10 group (P>0.05). In conclusion, TC-loaded SFM showed more bone formation than SFM without TC, and the amount of new bone formation was dependent on TC concentrations.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea

    Hyun Seok & Seong-Gon Kim

  2. Sericultural and Apicultural Materials Division, National Academy of Agricultural Science, Suwon, Korea

    HaeYong Kweon, You-Young Jo & Kwang-Gill Lee

  3. Gangneung Center, Korea Basic Science Institute, Gangneung, Korea

    Tae Yeon Kang & Weon-Sik Chae

  4. Department of Pathology, College of Medicine, Hallym University, Chuncheon, Korea

    Soo-Kee Min

  5. Department of Dentistry, College of Medicine, Hallym University, Chuncheon, Korea

    Jang-Hoon Ahn, Jun-Woo Park & Dong-Ju Choi

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  1. Hyun Seok
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Correspondence to Seong-Gon Kim.

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Seok, H., Kim, SG., Kweon, H. et al. Comparison of different concentrations of tetracycline-loaded silk fibroin membranes on the guided bone regeneration in the rat calvarial defect model. Tissue Eng Regen Med 11, 476–482 (2014). https://doi.org/10.1007/s13770-014-9057-3

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  • DOI: https://doi.org/10.1007/s13770-014-9057-3

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