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Determination of the optimal condition for ethylmethane sulfonate-mediated mutagenesis in a Korean commercial rice, Japonica cv. Dongjin

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Abstract

Ethylmethane sulfonate (EMS) mutagenesis is a powerful hunting tool to seek novel players for improving agromonic traits. Together with rapid evolution of the next-generation sequencing techniques, the EMS mutagenesis has been revaluated for its utilization to breed crops in practical agriculture and to study functions of key players in valuable agronomic traits. In this study, we systematically investigated conditions for EMS mutagenesis in Dongjin (Oryza sativa, Japonica) rice plants to make a mutant population. Since the EMS mutagenesis depends on target tissue, EMS concentration and EMS exposure time, we fixed the EMS exposure time as 13 h and treated germinating seeds with various levels of EMS dosage (from 0.25 to 2% EMS concentration). EMS treatment clearly showed negative biological influences including low germination and abnormal seedling development of Dongjin rice plants. Based on the standard of about 50% lethal dose, 0.75 and 1% EMS dosage for 13 h was finally selected as the optimal conditions for EMS mutagenesis of Dongjin germinating seeds.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea, Ministry of Education (NRF-2014R1A6A3A04053795 and NRF-2010-359-F00004), by the International Research and Development Program of the National Research Foundation of Korea, Ministry of Science, ICT and Future Planning (NRF-2015K1A3A1A21000237) and by the Rural Development Administration under the Next-Generation BioGreen 21 Program (PJ011829012016).

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Author notes

  1. D.-K. Lee and Y. S. Kim contributed equally to this work as co-first authors.

  2. D.-K. Lee and J.-K. Kim contributed equally to this work as co-corresponding authors.

Authors and Affiliations

  1. Graduate School of International Agricultural Technology and Crop Biotechnology Institute/GreenBio Science and Technology, Seoul National University, Pyeongchang, 25354, Korea

    Dong-Keun Lee, Youn Shic Kim & Ju-Kon Kim

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  1. Dong-Keun Lee
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  2. Youn Shic Kim
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Correspondence to Dong-Keun Lee or Ju-Kon Kim.

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Lee, DK., Kim, Y.S. & Kim, JK. Determination of the optimal condition for ethylmethane sulfonate-mediated mutagenesis in a Korean commercial rice, Japonica cv. Dongjin. Appl Biol Chem 60, 241–247 (2017). https://doi.org/10.1007/s13765-017-0273-0

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