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Two base mutations of a putative LacI-family transcriptional regulator, SCO7554, reverse the overexpression effect on the antibiotic pigment production in Streptomyces coelicolor

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Abstract

The SCO7554 protein, a putative LacI-family transcriptional regulator, is important in the antibiotic pigment production in Streptomyces coelicolor M130. A single mutation at the 4th amino acid from asparagine to serine reversed the SCO7554 effect on the antibiotic pigment production in an overexpression experiment. Additional mutation at the 276th amino acid from valine to alanine intensified this effect, and the overexpression of the double mutant SCO7554 increased the production of antibiotics, whereas the overexpression of SCO7554 itself decreased the antibiotic productions. This observation suggested the functional importance of the N-terminal region of a putative LacI-family transcriptional regulator, SCO7554 protein, even though it was not located on any known functional domain.

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Correspondence to Tae-Jong Kim or Joo-Won Suh.

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Meng, L., Yang, S.H., Kim, TJ. et al. Two base mutations of a putative LacI-family transcriptional regulator, SCO7554, reverse the overexpression effect on the antibiotic pigment production in Streptomyces coelicolor . J Korean Soc Appl Biol Chem 56, 99–101 (2013). https://doi.org/10.1007/s13765-012-2206-2

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  • DOI: https://doi.org/10.1007/s13765-012-2206-2

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