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Removal of anthropogenic lead pollutions by a potent Bacillus species AS2 isolated from geogenic contaminated site

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Abstract

A total of 422 bacterial isolates were obtained from the lead (Pb) ore in north-eastern Iran. The Pb tolerances of these strains were studied using microbroth serial dilution approach and 35 strains could grow up to 3250 ppm Pb concentration. Of these strains, 10 of them represented qualitatively high levels of Pb adsorption and were selected for quantitative studies. Strain AS2 which is phylogenetically related to genus Bacillus showed the highest level of Pb remediation. The effects of different factors, including pH, initial Pb concentration, temperature and inoculum size, were studied on the remediation process. Pb remediation capacity was reached at 74.5 mg/g (99.5 % of initial Pb) at pH 4.5, temperature 30 °C, inoculum size 1.0 % (v/v) and an initial Pb concentration of 500 ppm after 24 h. Pb desorption capacity of strain was 66 %. The novel isolate could remove 98 % of Pb from the contaminated industrial wastes after 24 h. Pb uptaking to the cell surface was proven using scanning electron microscopic micrograph and energy-dispersive X-ray spectroscopy analysis. Most Pb removal efficiency was observed in the active cell culture as compared to the inactive cell and extracellular polymeric substances. The novel strain represents a good candidate for removal of environmental anthropogenic Pb pollutions.

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Acknowledgments

This work was supported by grant from Ferdowsi University of Mashhad (23889/3).

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Authors and Affiliations

  1. Department of Geology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

    A. Cephidian & M. H. Mahmudy Gharaie

  2. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, P.O. Box: 91775-1436, Mashhad, Iran

    A. Makhdoumi & M. Mashreghi

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  1. A. Cephidian
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  2. A. Makhdoumi
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  3. M. Mashreghi
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  4. M. H. Mahmudy Gharaie
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Correspondence to A. Makhdoumi.

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Cephidian, A., Makhdoumi, A., Mashreghi, M. et al. Removal of anthropogenic lead pollutions by a potent Bacillus species AS2 isolated from geogenic contaminated site. Int. J. Environ. Sci. Technol. 13, 2135–2142 (2016). https://doi.org/10.1007/s13762-016-1023-2

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  • DOI: https://doi.org/10.1007/s13762-016-1023-2

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