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Effects of two plant growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase on oat growth in petroleum-contaminated soil

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Abstract

Petroleum is potentially toxic to living organisms, and there are worldwide efforts to develop methods for bioremediation of petroleum-contaminated soils. Phytoremediation is an effective method to reduce the concentration of petroleum in soils, and plant growth-promoting rhizobacteria (PGPR) play an important role in the phytoremediation. Two PGPR, Pseudomonas aeruginosa SLC-2 and Serratia marcescens BC-3, were isolated from the rhizophere of Echinochloa grown in petroleum-contaminated soil. These isolates showed capacities for 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, indole acetic acid production, siderophore synthesis, and the degradation of petroleum. The ACC deaminase activity of SLC-2 and BC-3 was 2.52 ± 0.03 μmol α-KA (mg Pr·h)−1 and 38.52 ± 0.37 μmol α-KA (mg Pr·h)−1, respectively. On the other hand, when the concentration of l-Trp increased, the IAA synthesis of BC-3 also increased, while the synthesis of SLC-2 did not change significantly. The ability of synthesized siderophore of SLC-2 was much higher than that of BC-3. The petroleum degradations of SLC-2 and BC-3 increased 4.78 and 7.36 %, respectively. The pot experiment of oat was performed to evaluate the plant growth-promoting abilities of SLC-2 and BC-3. Compared with non-inoculated controls, the height and fresh weight of stems increased (23.64 and 42.57) % and (16.98 and 28.3) %, respectively, whereas the length and fresh weight of roots also increased (10.34 and 20.84) and (24.13 and 43.11) %, respectively, when inoculated with SLC-2 and BC-3. The results indicated that P. aeruginosa SLC-2 and S. marcescens BC-3 can serve as promising microbes for increasing plant growth in petroleum-contaminated soil to improve the phytoremediation efficiency.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 31170479), the Natural Science Foundation of Heilongjiang province, China (Grant No. C201142), Programs for Science and Technology Development of Heilongjiang Province, China (Grant No. GC12B304), the Graduate Innovation Fund of Harbin Normal University (HSDBSCX2013-04), and Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Heilongjiang Province (2010TD10) and Harbin Normal University (KJTD2011-2).

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  1. Key Laboratory of Molecular and Cytogenetics, Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China

    J. L. Liu, B. M. Xie, X. H. Shi, J. M. Ma & C. H. Guo

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  1. J. L. Liu
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  2. B. M. Xie
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Correspondence to C. H. Guo.

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Liu, J.L., Xie, B.M., Shi, X.H. et al. Effects of two plant growth-promoting rhizobacteria containing 1-aminocyclopropane-1-carboxylate deaminase on oat growth in petroleum-contaminated soil. Int. J. Environ. Sci. Technol. 12, 3887–3894 (2015). https://doi.org/10.1007/s13762-015-0798-x

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  • DOI: https://doi.org/10.1007/s13762-015-0798-x

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