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Microbial community dynamics in the two-stage anaerobic digestion process of two-phase olive mill residue

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Abstract

The microbial communities in a two-stage anaerobic digestion process treating olive mill “solid” residues were studied by molecular identification techniques. The microbial species identification in the hydrolytic-acidogenic step and in the methanogenic step was carried out by polymerase chain reaction amplification of 16S ribosomal RNA genes, denaturing gradient gel electrophoresis, cloning, and sequencing. This study revealed that Firmicutes (from 31.1 to 61.1 %, average 42.1 %) mainly represented by Clostridiales, and the Chloroflexi (from 29.4 to 53.3 %, average 47.35 %) were the most abundant species for the hydrolytic-acidogenic reactor. Other microorganisms such as Gamma-Proteobacteria (Pseudomonas species as the major representative; from 3.9 to 9.7 %; average 5.7 %), Actinobacteria (from 1.0 to 10.2 %; average 4.6 %) and Bacteroidetes (from 1.1 to 3.1 %; average 1.7 %) were also detected. The methanogenic communities detected in the methanogenic reactor were mainly represented by members of the obligate acetotrophic methanogenic genus Methanosaeta. Methanosaeta was the crucial Archaea to obtain a high methane yield in the methanogenic stage.

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Acknowledgments

The authors wish to express their gratitude to the Spanish “Ministerio de Educación y Ciencia” (Project REN 2001-0472/TECNO) and “Junta de Andalucía” for providing financial support and to the contracts JAE-Doc from “Junta para la Ampliación de Estudios del Consejo Superior de Investigaciones Científicas (CSIC)” co-financed by the European Social Funds.

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Authors and Affiliations

  1. Instituto de la Grasa (CSIC), Avenida Padre García Tejero 4, 41012, Sevilla, Spain

    B. Rincón & R. Borja

  2. Instituto de Recursos Naturales y Agrobiología (CSIC), Apartado 1052, 41080, Sevilla, Spain

    M. C. Portillo & J. M. González

Authors
  1. B. Rincón
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  2. M. C. Portillo
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  3. J. M. González
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  4. R. Borja
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Correspondence to B. Rincón.

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Rincón, B., Portillo, M.C., González, J.M. et al. Microbial community dynamics in the two-stage anaerobic digestion process of two-phase olive mill residue. Int. J. Environ. Sci. Technol. 10, 635–644 (2013). https://doi.org/10.1007/s13762-013-0290-4

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  • DOI: https://doi.org/10.1007/s13762-013-0290-4

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