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Degradation of native wheat straw lignin by Streptomyces viridosporus T7A

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Abstract

Lignin is one of the major contributing factors toward the recalcitrance of lignocellulosic biomass. Understanding the process of lignin degradation in natural biological processes will provide useful information to develop novel biomass conversion technologies. Functional group changes in the lignin entities during the process may contribute to the cellulose degradation (utilization) by the microorganisms. In this study, compositional and advanced Fourier transform infrared, pyrolysis gas chromatography/mass spectrometry and 13C cross polarization/magic angle spinning nuclear magnetic resonance analysis were performed to explore the mechanism of biodegradation of wheat straw by Streptomyces viridosporus T7A. The results indicated that S. viridosporus T7A removed lignin and hemicelluloses as indicated by the increased carbohydrate/lignin ratio. Significant modification of carbonyl and methoxyl groups in the complex lignin structure was also evident. Most importantly, the quantitative results showed that lignin degradation was featured by deduction of guaiacyl unit. The results provide new insight for understanding lignin degradation by bacteria.

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Acknowledgments

Authors also would like to thank Dr. Lee Deobald, University of Idaho for kindly providing the S. viridosporus T7A strain for the study and Allan Gao, Dr. Jim O’Fallon for their sincere help in this manuscript. The Nuclear Magnetic Resonance center, Washington State University, is gratefully acknowledged for providing NMR facilities.

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Author notes

  1. D. Singh

    Present address: Edeniq Inc., Visalia, CA, 93291, USA

Authors and Affiliations

  1. Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman, WA, 99163, USA

    J. Zeng, D. Singh, D. D. Laskar & S. Chen

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  1. J. Zeng
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  4. S. Chen
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Correspondence to S. Chen.

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Zeng, J., Singh, D., Laskar, D.D. et al. Degradation of native wheat straw lignin by Streptomyces viridosporus T7A. Int. J. Environ. Sci. Technol. 10, 165–174 (2013). https://doi.org/10.1007/s13762-012-0085-z

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  • DOI: https://doi.org/10.1007/s13762-012-0085-z

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