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Toxicity of an Annonin-Based Commercial Bioinsecticide Against Three Primary Pest Species of Stored Products

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Abstract

The effects of a bioinsecticide formulation based on extract of Annona squamosa L. (Annonaceae) containing 10,000 mg L−1 of acetogenin annonin as the main active ingredient were investigated against three primary pest species of stored grains in Brazil [maize weevil Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), Mexican bean weevil Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomelidae: Bruchinae), and cowpea weevil Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae)] by means of residual contact bioassays. In a concentration-dependent manner, the annonin-based commercial bioinsecticide caused significant adult mortality of C. maculatus (LC50 = 6890 μL kg−1), S. zeamais (LC50 = 2781 μL kg−1), and Z. subfasciatus (LC50 = 2120 μL kg−1) after 120 h of residual contact exposure. In addition to acute toxicity, the tested bioinsecticide also promoted a significant reduction of the number of eggs laid by females of C. maculatus (EC50 = 5949.7 μL kg−1) and Z. subfasciatus (EC50 = 552.7 μL kg−1). Moreover, the bioinsecticide significantly reduced the number of emerged insects (F1 generation) of C. maculatus (EC50 = 2763.0 μL kg−1), S. zeamais (EC50 = 1380.8 μL kg−1), and Z. subfasciatus (EC50 = 561.5 μL kg−1). The bioinsecticide also reduced the percentage of damaged grains for the three pest species studied, and its grain-protectant properties are comparable to or superior in efficacy in relation to a diatomaceous earth-based insecticide (Insecto® at 1000 mg kg−1) used as a positive control. Thus, this standardized formulation has promising bioactivity against stored insect species and can be a useful component for IPM of stored grains in Brazil and elsewhere.

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Acknowledgments

The authors thank the São Paulo State Research Foundation (FAPESP-grant number 2010/52638-0) and the National Council for Scientific and Technological Development (CNPq-grant number 140651/2013-6) for the financial support and scholarships granted.

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Authors and Affiliations

  1. EPAGRI/CEPAF, Av. Servidão Ferdinando Tusset S/N, São Cristóvão, CEP 89801-970, Chapecó, SC, Brasil

    L P Ribeiro

  2. Department of Entomology and Acarology, “Luiz de Queiroz” College of Agriculture, Univ of São Paulo (ESALQ/USP), Piracicaba, São Paulo, Brasil

    O Z Zanardi, G L P Gonçalves, T F Ansante, P T Yamamoto & J D Vendramim

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  1. L P Ribeiro
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  2. O Z Zanardi
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  3. G L P Gonçalves
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  4. T F Ansante
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  5. P T Yamamoto
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  6. J D Vendramim
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Correspondence to L P Ribeiro.

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Edited by Lessando Moreira Gontijo – UFV

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Ribeiro, L.P., Zanardi, O.Z., Gonçalves, G.L.P. et al. Toxicity of an Annonin-Based Commercial Bioinsecticide Against Three Primary Pest Species of Stored Products. Neotrop Entomol 47, 145–151 (2018). https://doi.org/10.1007/s13744-017-0510-6

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  • DOI: https://doi.org/10.1007/s13744-017-0510-6

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