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Bioinspired synthesis, characterization and antifungal activity of enzyme-mediated gold nanoparticles using a fungal oxidoreductase

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Abstract

The development of efficient cell-free systems of nanoparticle synthesis using microbial enzymes is a growing field of biological and green chemistry for the supportable improvement in nano-biotechnology. In the present study, we established a cell-free system for producing gold nanoparticles (AuNPs) using a fungal oxidoreductase named sulfite oxidoreductase purified to homogeneity from Fusarium oxysporum. The enzyme was purified by ultrafiltration followed by anion exchange chromatography on DEAE Sephadex A-50 gel, and its molecular weight was determined by gel filtration chromatography on Sephacryl S-300 gel. The purified enzyme had a molecular weight of 346 kDa. It was composed of three subunits of 176, 94 and 76 kDa. Purified enzyme was successfully used for production of gold nanoparticles in a cell-free system. Synthesized gold nanoparticles showed the highest absorbance at 520 nm wavelength as shown by UV–visible spectroscopy. They were spherical in shape with an average size of 20 nm as determined by scanning and transmission electron microscopy and dynamic light scattering. Assessment of the antifungal properties of synthesized nanoparticles by disk diffusion method indicated a potent growth inhibitory activity against all tested human pathogenic yeasts and molds by inhibition zones ranged from 10 to 18 mm. Taken together, our enzymatically established method of nanoparticle synthesis using a purified sulfite oxidoreductase of F. oxysporum can be considered as an efficient tool for generating harmless bioactive gold nanoparticles with potential applications in biology, medicine and industry.

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Acknowledgments

This work was supported financially by the Pasteur Institute of Iran (Project No. 766).

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Authors and Affiliations

  1. Department of Mycology, Pasteur Institute of Iran, Tehran, 13164, Iran

    Mohammadhassan Gholami-Shabani & Mehdi Razzaghi-Abyaneh

  2. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, 13164, Iran

    Mohammadhassan Gholami-Shabani, Afshin Imani, Zeynab Gholami-Shabani & Azim Akbarzadeh

  3. Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-331, Iran

    Masoomeh Shams-Ghahfarokhi

  4. Faculté de Pharmacie, Université de Picardie Jules-Verne, 80000, Amiens, France

    Arezoo Pazooki

  5. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Gholamhossein Riazi

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  1. Mohammadhassan Gholami-Shabani
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Correspondence to Mehdi Razzaghi-Abyaneh.

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Gholami-Shabani, M., Imani, A., Shams-Ghahfarokhi, M. et al. Bioinspired synthesis, characterization and antifungal activity of enzyme-mediated gold nanoparticles using a fungal oxidoreductase. J IRAN CHEM SOC 13, 2059–2068 (2016). https://doi.org/10.1007/s13738-016-0923-x

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  • DOI: https://doi.org/10.1007/s13738-016-0923-x

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