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Fluorophore-tagged superparamagnetic iron oxide nanoparticles as bimodal contrast agents for MR/optical imaging

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Abstract

A stable bimodal contrast agent for both MRI and fluorescence imaging was prepared based on fluorescent superparamagnetic iron oxide nanoparticles. In order to increase the stability of the product in a physiological medium, the designed route for preparation of this bimodal contrast agent is based on covalent linkages. Superparamagnetic magnetite nanoparticles were prepared by a coprecipitation method in polyol media, and then the surface of nanoparticles was modified with 3-aminopropyltriethoxysilane (APTES) to introduce reactive amine groups on the surface of nanoparticles. The hybrid nanoparticles were obtained by conjugating rhodamine B to APTES-modified superparamagnetic Fe3O4 nanoparticles through amide linkage. Longitudinal (r 1) and transverse (r 2) proton relaxivities and their ratio (r 2/r 1) obtained for bimodal contrast agent in different magnetic fields show their higher efficiency as T 2 contrast agent for MRI in comparison with commercially available magnetite-based contrast agents. Moreover, these nanoparticles can be followed up by fluorescence imaging. This study shows that rhodamine B-tagged iron oxide nanoparticles are well suited as a contrast agent for dual modality magnetic resonance and fluorescence imaging.

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Acknowledgments

This work was supported by Walloon Region (program First spin-off), Fond National de la Recherche Scientifique (FNRS), UIAP VII and ARC Programs of the French Community of Belgium. Also, the financial support of the Iranian Nanotechnology Society is gratefully acknowledged. The support and sponsorship accorded by COST Actions D38 and TD1004 are kindly acknowledged. The authors thank the Center for Microscopy and Molecular Imaging (CMMI, supported by the European Regional Development Fund and the Walloon Region).

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Chemistry, University of Mahaghegh Ardabili, Ardabil, Iran

    Parvin Eghbali

  2. North-West Institute of Science and Technology, Malek Ashtar University of Technology, Urmia, Iran

    Hassan Fattahi & Yones Mosaei Oskoei

  3. Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Avenue Maistriau 19, 7000, Mons, Belgium

    Sophie Laurent & Robert N. Muller

  4. Center for Microscopy and Molecular Imaging (CMMI), Rue Adrienne Bolland 8, 6041, Gosselies, Belgium

    Sophie Laurent & Robert N. Muller

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  1. Parvin Eghbali
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  2. Hassan Fattahi
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Correspondence to Hassan Fattahi.

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Eghbali, P., Fattahi, H., Laurent, S. et al. Fluorophore-tagged superparamagnetic iron oxide nanoparticles as bimodal contrast agents for MR/optical imaging. J IRAN CHEM SOC 13, 87–93 (2016). https://doi.org/10.1007/s13738-015-0715-8

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  • DOI: https://doi.org/10.1007/s13738-015-0715-8

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