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Blood coagulating effect of marigold (Tagetes erecta L.) leaf and its bioactive compounds

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Abstract

Marigold (Tagets erecta L.), being an ornamental plant, posses medicinal properties like antimicrobial, insecticidal, wound healing and blood coagulation properties. Our recent works mainly focus on blood clotting activity of marigold leaf extracts. Blood clotting activity of leaf extract was examined using prothrombin time (PT) test by Owren method. The optimization of marigold leaf coagulating activity at different concentration of leaf extract and blood was done with Response surface method (RSM). Experiments showed that there was decrease in coagulation time with increase in extract concentration. Marigold leaves extract has shown very good blood coagulation property in lesser quantity i.e., in the range of μl. Also, phenolic compounds (like gallic acid, scopoletin, ferulic acid, quercecetin) were identified and quantified, which shows that because of presence of them, marigold leaf extract shows coagulation property. Antifungal, antibacterial and antioxidant activity are also analyzed. From the above findings, it can be concluded that along with antibacterial, antifungal and antioxidant properties, marigold leave extracts are also having very good coagulation property; so, in further research it can be developed as an antiseptic with coagulation property and can be developed as a potent product in the field of medicine and ointment.

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Acknowledgments

SR and ND are acknowledging Mrs. Maya Majumdar, Lucknow, India for giving the basic idea about the medicinal properties of marigold. Authors are acknowledging Jai Prakash University, Chapra, Bihar, India for providing technical support to carry out the work. Authors are acknowledging the partial funding provided by Veer Kunwar Singh Memorial Trust, Chapra, Bihar, India to complete this project-VKSMT/SN/NFNA/0011. Authors are acknowledging Department of Biotechnology (DBT, India) for the funding with grant number – BT/PR10414/PFN/20/961/2014.

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    Authors and Affiliations

    1. Nano-food Research Group, Instrumental and Food Analysis Laboratory, Industrial Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, India

      Nandita Dasgupta, Shivendu Ranjan & Chidambaram Ramalingam

    2. Research Wing, Veer Kunwar Singh Memorial Trust, Chapra, Bihar, India, 841301

      Shivendu Ranjan

    3. Department of Zoology, Jai Prakash University, Chapra, Bihar, India

      Madhu Shree

    4. Industrial Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore, India

      M.A. Arabi Mohammed Saleh

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    1. Nandita Dasgupta
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    Correspondence to Shivendu Ranjan or Chidambaram Ramalingam.

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    The authors received ethical clearance (as needed) from Veer Kunwar Singh Memorial Trust, Chapra, India with the reference number VKSMNT/SN/2015/RES/03.

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    Nandita Dasgupta and Shivendu Ranjan contributed equally to this work.

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    Dasgupta, N., Ranjan, S., Shree, M. et al. Blood coagulating effect of marigold (Tagetes erecta L.) leaf and its bioactive compounds. Orient Pharm Exp Med 16, 67–75 (2016). https://doi.org/10.1007/s13596-015-0200-z

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