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Antidiabetic potential of Rourea minor (Gaertn.) root in streptozotocin—induced diabetic rats

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Abstract

The present investigation aims to examine the antihyperglycemic potential of Rourea minor (Gaertn.) root in normal and streptozotocin (STZ) induced diabetic rats. The root of Rourea minor (Gaertn.) was extracted using ethanol and water and tested for acute toxicity by up and down staircase method. The aqueous Rourea minor (Gaertn.) water (RMW) extract and ethanolic Rourea minor (Gaertn.) extract (RME) at 200 and 400 mg/kg bodyweight doses were screened for blood glucose lowering capacity in normal and STZ induced diabetic animals; the blood samples were collected from the retro orbital plexus and analyzed for serum glucose (SG) level. Glibenclamide (GLB) was used as a standard. The oral glucose tolerance test (OGTT) was carried out in normal as well as STZ induced diabetic rats whereas, insulin tolerance test was carried out in diabetic rats. At the end of 15 days treatment, serum triglyceride (STG), total cholesterol (STC) and HDL-cholesterol (HDL-c) were assayed by auto analyzer. The results of acute toxicity showed that the animals had good tolerance to single doses of RME/RMW (as high as 3 g/kg) and were non-lethal. The glucose tolerance test in normal rats showed that treatment of RME, RMW (both 400 mg/kg), RME (200 mg/kg) and GLB (0.5 mg/kg) exhibited significant reduction (p < 0.001) in SG level over a period of 120 min of oral administration of root extract. After 15 days of daily oral administration of the extracts to STZ induced diabetic rats, higher dose of RME could reduce hyperglycemia to an extent of maximum 43.14 %. Also the ethanolic extract was more effective in reducing the SG level (OGTT in STZ induced diabetic rats) with improved glucose tolerance compared to an aqueous extract. Both the doses of RME and RMW exhibited significant reduction (P < 0.001) in all tested lipid parameters as compared to diabetic control rats and restored them to nearly-normal values.

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Acknowledgments

The authors are thankful to Sri. H. V. Dambal, President Soniya Education Trust’s College of Pharmacy, Dharwad for providing the necessary facilities to carry out the study.

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  1. Department of Pharmacology, Soniya Education Trust’s College of Pharmacy, Dharwad, Karnataka, India

    Preeti Kulkarni, Vishal Patel, S. T. Shukla, Ankur Patel & Venkatrao Kulkarni

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  1. Preeti Kulkarni
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  2. Vishal Patel
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  3. S. T. Shukla
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  4. Ankur Patel
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  5. Venkatrao Kulkarni
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Correspondence to S. T. Shukla.

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Kulkarni, P., Patel, V., Shukla, S.T. et al. Antidiabetic potential of Rourea minor (Gaertn.) root in streptozotocin—induced diabetic rats. Orient Pharm Exp Med 14, 69–76 (2014). https://doi.org/10.1007/s13596-013-0129-z

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