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Apis mellifera hemocytes generate increased amounts of nitric oxide in response to wounding/encapsulation

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A Correction to this article was published on 16 July 2019

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Abstract

Apis mellifera populations are being threatened by several pathogens and parasitosis. Several authors have proposed that honey bee colonies may suffer from a compromised immune system leading to colony loss. This is why the study of A. mellifera immune system has become a topic of pressing concern. Nitric oxide (NO) is a signaling and immune effector molecule that has been proposed as a key molecule in invertebrate immunity, and that plays a part in A. mellifera cellular defenses. This paper deals with NO participation in the response to wounding/encapsulation challenge in A. mellifera fifth instar (L5) larvae. Challenging A. mellifera L5 larvae with nylon implants enhanced NO production and spreading in granulocyte-like hemocytes and increased the number of this NO-producing hemocyte type. However, AmNOS expression levels were not influenced by the insult. These results reveal that NO participates in the wound healing/encapsulation response as a signal molecule, possibly by the activation of a constitutively expressed AmNOS in honey bees.

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Change history

  • 16 July 2019

    This correction stands to correct the last sentence found in the caption of Figure 2 where it reads, ���Three independent experiments were performed and a total of 18 larvae per treatment were analyzed.���

  • 16 July 2019

    This correction stands to correct the last sentence found in the caption of Figure 2 where it reads, ���Three independent experiments were performed and a total of 18 larvae per treatment were analyzed.���

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Acknowledgments

This research was supported by PICT-2012-0594 granted by the “Agencia Nacional de Promoción Científica y Tecnológica” to M.E. The authors would like to thank CONICET and the UNMdP. Pedro Negri is a doctoral fellow from CONICET, Argentina.

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Authors and Affiliations

  1. Laboratorio de Artrópodos, Facultad de Cs Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Mar del Plata, Argentina

    Pedro Negri, Matias Maggi, Nicolas Szawarski & Martin Eguaras

  2. Instituto de Investigaciones Biológicas-CONICET, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina

    Lorenzo Lamattina

  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Sarmiento, Argentina

    Pedro Negri, Matias Maggi, Lorenzo Lamattina & Martin Eguaras

  4. Laboratorio de Biología Molecular, Fares Taie Instituto de Análisis, Mar del Plata, Argentina

    Silvina Quintana

  5. Funes 3350, Facultad de Cs Exactas y Naturales, nivel 0, Mar del Plata, PC 7600, Argentina

    Pedro Negri

Authors
  1. Pedro Negri
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  2. Silvina Quintana
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  3. Matias Maggi
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  4. Nicolas Szawarski
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  5. Lorenzo Lamattina
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  6. Martin Eguaras
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Corresponding author

Correspondence to Pedro Negri.

Additional information

Manuscript editor: Klaus Hartfelder

Les hémocytes d’Apis mellifera produisent des quantités accrues d’oxyde nitrique en réponse à une blessure/encapsulation

Abeille domestique/larve/défense immunitaire/réaction de défense

Als Antwort auf eine Verwundungs/Einschlussreaktion produzieren die Hämozyten von Apis mellifera erhöhte Mengen an Stickoxid

Apis mellifera/Stickoxid/Verwundung/Einschlussreaktion

M. Eguaras and L. Lamattina contributed equally as senior authors.

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Negri, P., Quintana, S., Maggi, M. et al. Apis mellifera hemocytes generate increased amounts of nitric oxide in response to wounding/encapsulation. Apidologie 45, 610–617 (2014). https://doi.org/10.1007/s13592-014-0279-0

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  • DOI: https://doi.org/10.1007/s13592-014-0279-0

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