Apidologie

, Volume 44, Issue 4, pp 467–480

Sublethal doses of imidacloprid decreased size of hypopharyngeal glands and respiratory rhythm of honeybees in vivo

Authors

    • Hellenic Institute of ApicultureHellenic Agricultural Organization ‘DEMETER’
  • Chrisovalantis Papaefthimiou
    • Laboratory of Animal Physiology, Department of ZoologySchool of Biology, Aristotle University
  • Leonidas Charistos
    • Hellenic Institute of ApicultureHellenic Agricultural Organization ‘DEMETER’
  • Taylan Dogaroglu
    • Mugla University, Science Faculty, Biology Department
  • Maria Bouga
    • Lab of Agricultural Zoology and EntomologyAgricultural University of Athens
  • Christina Emmanouil
    • Lab of Agricultural Zoology and EntomologyAgricultural University of Athens
  • Gerard Arnold
    • Laboratoire Evolution, Génomes, Spéciation, CNRS UPR 9034, 91198 Gif-sur-YvetteFrance and Université Paris-Sud 11
Original article

DOI: 10.1007/s13592-013-0199-4

Cite this article as:
Hatjina, F., Papaefthimiou, C., Charistos, L. et al. Apidologie (2013) 44: 467. doi:10.1007/s13592-013-0199-4

Abstract

Most studies that have shown negative sublethal effects of the pesticide imidacloprid on honeybees concern behavioral effects; only a few concern physiological effects. Therefore, we investigated sublethal effects of imidacloprid on the development of the hypopharyngeal glands (HPGs) and respiratory rhythm in honeybees fed under laboratory conditions. We introduced newly emerged honeybees into wooden mesh-sided cages and provided sugar solution and pollen pastry ad libitum. Imidacloprid was administered in the food: 2 μg/kg in the sugar solution and 3 μg/kg in the pollen pastry. The acini, the lobes of the HPGs of imidacloprid-treated honeybees, were 14.5 % smaller in diameter in 9-day-old honeybees and 16.3 % smaller in 14-day-old honeybees than in the same-aged untreated honeybees; the difference was significant for both age groups. Imidacloprid also significantly affected the bursting pattern of abdominal ventilation movements (AVM) by causing a 59.4 % increase in the inter-burst interval and a 56.99 % decrease in the mean duration of AVM bursts. At the same time, the quantity of food consumed (sugar solution and pollen pastry) per honeybee per day was the same for both treated and untreated honeybees.

Keywords

imidacloprid honeybee hypopharyngeal gland respiratory rhythm

Copyright information

© INRA, DIB and Springer-Verlag France 2013