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Genetic structure of Chinese Apis dorsata population based on microsatellites

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Abstract

We investigated the genetic structure of Chinese Apis dorsata population based on DNA microsatellites. The results show that populations from different geographical regions are significantly differentiated. Furthermore, the A. dorsata population from Hainan Island very probably diverged from that of the China mainland and also exhibits a lower level of genetic diversity, which is probably the result of founder effects and genetic drift. The results provide useful information for effective management and conservation of A. dorsata in China, particularly with regard to the population of Hainan Island.

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References

  • Arias, M.C., Sheppard, W.S. (2005) Phylogenetic relationships of honey bees (Hymenoptera: Apinae: Apini) inferred from nuclear and mitochondrial DNA sequence data. Mol. Phylogenet. Evol. 37, 25–35

    Article  PubMed  CAS  Google Scholar 

  • Berghoff, S.M., Kronauer, D.J.C., Edwards, K.J., Franks, N.R. (2008) Dispersal and population structure of a New World predator, the army ant Eciton burchellii. J. Evol. Biol. 21, 1125–1132

    Article  PubMed  CAS  Google Scholar 

  • Corlett, R.T. (2011) Honeybees in natural ecosystems. In: Hepburn, H.R., Radloff, S.E. (eds.) Honeybees of Asia, pp. 215–226. Springer-Verlag, Berlin

    Chapter  Google Scholar 

  • Estoup, A., Solignac, M., Harry, M., Cornuet, J.M. (1993) Characterization of (GT)n and (CT)n microsatellites in two insect species: Apis mellifera and Bombus terrestris. Nucleic Acids Res. 21, 1427

    Article  PubMed  CAS  Google Scholar 

  • Estoup, A., Solignac, M., Cornuet, J.M. (1994) Precise assessment of the number of patrilines and of genetic relatedness in honeybee colonies. Proc. R. Soc. Lond. B 258, 1–7

    Article  CAS  Google Scholar 

  • Estoup, A., Solignac, M., Cornuet, J.M., Goudet, J., Scholl, A. (1996) Genetic differentiation of continental and island populations of Bombus terrestris (Hymenoptera: Apidae) in Europe. Mol. Ecol. 5, 19–31

    Article  PubMed  CAS  Google Scholar 

  • Frankham, R. (1997) Do island populations have less genetic variation than mainland populations? Heredity 78, 311–327

    Article  PubMed  Google Scholar 

  • Gao, P.F., Zhao, H.T., Zhang, C.X., Jiang, Y.S. (2008) Phylogeny of different geographic populations Apis cerana in China based on mtDNA cytochrome b gene sequences. Acta Zoologica Sinica 54, 1005–1013 [in Chinese]

    CAS  Google Scholar 

  • Goldstein, D.B., Schlötterer, C. (1999) Microsatellites: evolution and applications. Oxford Univ. Press., Oxford

    Google Scholar 

  • Goudet, J. (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995)

  • Groom, S.V.C., Schwarz, M.P. (2011) Bees in the Southwest Pacific: origins, diversity and conservation. Apidologie 42(6): 759–770

    Article  Google Scholar 

  • Hepburn, H.R., Radloff, S.E. (2011) Biogeography. In: Hepburn, H.R., Radloff, S.E. (eds.) Honeybees of Asia, pp. 62–63. Springer-Verlag, Berlin

    Chapter  Google Scholar 

  • Insuan, S., Deowanish, S., Klinbunga, S., Sittipraneed, S., Sylvester, H.A., Wongsiri, S. (2007) Genetic differentiation of the giant honey bee (Apis dorsata) in Thailand analyzed by mitochondrial genes and microsatellites. Biochem. Genet. 45, 345–361

    Article  PubMed  CAS  Google Scholar 

  • Jiang, Y.S., Zhao, H.T., Jiang, J.B., Cao, G.Q., Zhang, G.X., Zhu, W.J., Guo, C.J. (2007) Studies on mtDNA tRNAleu ∼ COII gene polymorphisms of Apis cerana distributed in different geographic areas in China. Scienfia Agricultura Sinica 40, 1535–1542 [in Chinese]

    CAS  Google Scholar 

  • Kaiser-Bunbury, C.N., Traveset, A., Hansen, D.M. (2010) Conservation and restoration of plant-animal mutualisms on oceanic islands. Perspectives in Plant Ecology, Evol. Syst. 12, 131–143

    Article  Google Scholar 

  • Kalinowski, S.T. (2004) Counting alleles with rarefaction: Private alleles and hierarchical sampling designs. Conserv. Genet. 5, 539–543

    Article  CAS  Google Scholar 

  • Kalinowski, S.T. (2005) HP-RARE 1.0: a computer program for performing rarefaction on measures of allelic richness. Mol. Ecol. Notes 5, 187–189

    Article  CAS  Google Scholar 

  • Kraus, F.B., Koeniger, N., Tingek, S., Moritz, R.F.A. (2005) Temporal genetic structure of a drone congregation area of the giant Asian honeybee (Apis dorsata). Naturwissenschaften 92, 578–581

    Article  PubMed  CAS  Google Scholar 

  • Kronauer, D.J.C., Berghoff, S.M., Powell, S., Denny, A.J., Edwards, K.J., Franks, N.R., Boomsma, J.J. (2006) A reassessment of the mating system characteristics of the army ant Eciton burchellii. Naturwissenschaften 93, 402–406

    Article  PubMed  CAS  Google Scholar 

  • Lo, N., Gloag, R.S., Anderson, D.L., Oldroyd, B.P. (2010) A molecular phylogeny of the genus Apis suggests that the Giant Honey Bee of the Philippines, A. breviligula Maa, and the Plains Honey Bee of southern India, A. indica Fabricius, are valid species. Syst. Entomol. 35, 226–233

    Article  Google Scholar 

  • Moilanen, A., Sundström, L., Pedersen, J.S. (2004) MATESOFT: a program for deducing paternal genotypes and estimating mating system statistics in haplodiploid species. Mol. Ecol. Notes 4, 795–797

    Article  Google Scholar 

  • Nath, S., Roy, P., John, M., Leo, R. (1994) Honey hunters and beekeepers of Tamil Nadu. A survey document. Keystone, Pondicherry, India

    Google Scholar 

  • Nei, M. (1987) Molecular evolutionary genetics. Columbia Univ. Press., New York

    Google Scholar 

  • Nei M., Tajima F., Tateno Y. (1983) Accuracy of estimated phylogenetic trees from molecular data. J. Mol. Evol. 19, 153–170

    Article  PubMed  CAS  Google Scholar 

  • Oldroyd, B.P., Nanork, P. (2009) Conservation of Asian honey bees. Apidologie 40, 296–312

    Article  Google Scholar 

  • Oldroyd, B.P., Wongsiri, S. (2006) Asian honey bees: biology, conservation, and human interactions. Harvard Univ. Press, Cambridge, Massachusetts

    Google Scholar 

  • Oldroyd, B.P., Smolenski, A.J., Cornuet, J.M., Wongsiri, S., Estoup, A., Rinderer, T.E., Crozier, R.H. (1996) Levels of polyandry and intracolonial genetic relationships in Apis dorsata (Hymenoptera: Apidae). Ann. Entomol. Soc. Am. 89, 276–283

    Google Scholar 

  • Ota T. (1993) DISPAN. Genetic distance and phylogenetic analysis. Pennsylvania State University. University Park, PA, USA

    Google Scholar 

  • Paar, J., Oldroyd, B.P., Huettinger, E., Kastberger, G. (2002) Drifting of workers in nest aggregations of the giant honeybee Apis dorsata. Apidologie 33, 553–561

    Article  Google Scholar 

  • Paar, J., Oldroyd, B.P., Huettinger, E., Kastberger, G. (2004) Genetic structure of an Apis dorsata population: the significance of migration and colony aggregation. J. Hered. 95, 119–126

    Article  PubMed  CAS  Google Scholar 

  • Partap, U. (2011) The pollination role of honeybees. In: Hepburn, H.R., Radloff, S.E. (eds.) Honeybees of Asia, pp. 227–255. Springer-Verlag, Berlin

    Chapter  Google Scholar 

  • Peakall, R., Smouse, P.E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6, 288–295

    Article  Google Scholar 

  • Rousset, F. (2008) GENEPOP ' 007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol. Ecol. Resour. 8, 103–106

    Article  PubMed  Google Scholar 

  • Ruttner, F. (1988) Biogeography and taxonomy of honeybees. Springer-Verlag, Berlin.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F., Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Smith, D.R. (1991) Mitochondrial DNA and honey bee biogeography. In: Smith, D.R. (eds.) Diversity in the Genus Apis, pp. 131–176. Westview Press, Boulder, CO

    Google Scholar 

  • Weir, B., Cockerham, C.C. (1984) Estimating F-statistics for the analysis of population structure. Evolution 38, 1358–1370

    Article  Google Scholar 

  • Widmer, A., Schmid-Hempel, P., Estoup, A., Scholl, A. (1998) Population genetic structure and colonization history of Bombus terrestris s.l. (Hymenoptera: Apidae) from the Canary Islands and Madeira. Heredity 81, 563–572

    Article  Google Scholar 

  • Wongsiri, S., Chanchao, C., Lekprayoon, C., Wattanasermkit, K., Deowanish, S., Leepitakrat, S. (2001) Honeybee diversity and management in the new millennium in Thailand. In: Proceedings of the 7th International Conference on Tropical Bees, pp. 9–14. Chiang Mai

  • Woyke, J., Wilde, J., Wilde, M., Sivaram, V., Cervancia, C., Nagaraja, N., Reddy, M. (2008) Comparison of defense body movements of Apis laboriosa, Apis dorsata dorsata and Apis dorsata breviligula honey bees. J. Insect Behav. 21, 481–494

    Article  Google Scholar 

  • Yang, G.H. (1984a) The survey of the resource of the Chinese honey bee, Zhongguo Yangfeng 3, 4–7 [in Chinese]

    Google Scholar 

  • Yang, G.H. (1984b) The survey of the resource of the Chinese honey bee II, Zhongguo Yangfeng 6, 16–19 [in Chinese]

    Google Scholar 

  • Zhao, H., Wang, L.R., Yuan, J.Y. (2007) Origin and time of Qiongzhou Strait. Marine Geology and Quaternary Geology 27, 33–40 [in Chinese]

    CAS  Google Scholar 

Download references

Acknowledgments

We gratefully acknowledge Hai-Ou Kuang, Jing-Lin Gao, Chuan-Quan Yang, Shao-Yu He, Xue-Wen Zhang, Jie Yang, and Xuan Chen for their help during sampling. We thank De-Fang Niu and Gang Zhang for technical support and Wen-Ting Wei for statistical support. This study was supported by the fund for Modern Agro-industry Technology Research System from the Ministry of Agriculture of the People’s Republic of China (CARS-45).

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Authors and Affiliations

  1. College of Animal Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China

    Lian-Fei Cao, Huo-Qing Zheng & Fu-Liang Hu

  2. Department of Zoology and Entomology, Rhodes University, Grahamstown, South Africa

    H. R. Hepburn

Authors
  1. Lian-Fei Cao
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  2. Huo-Qing Zheng
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  3. Fu-Liang Hu
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  4. H. R. Hepburn
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Correspondence to Fu-Liang Hu.

Additional information

Structure génétique de la population chinoise d’ Apis dorsata , basée sur les microsatellites

Apis dorsata / structure génétique / Chine / microsatellites / diversité génétique

Genetische Struktur von Apis dorsata Populationen in China auf der Basis von Mikrosatelliten

Apis dorsata / genetische Struktur / China / Mikrosatelliten

Manuscript editor: Marina Meixner

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Cao, LF., Zheng, HQ., Hu, FL. et al. Genetic structure of Chinese Apis dorsata population based on microsatellites. Apidologie 43, 643–651 (2012). https://doi.org/10.1007/s13592-012-0139-8

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  • DOI: https://doi.org/10.1007/s13592-012-0139-8

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