Apidologie

, Volume 43, Issue 2, pp 128–138

RNA interference in honeybees: off-target effects caused by dsRNA

Authors

    • Institut für BiologieMartin-Luther-Universität Halle-Wittenberg
  • Robin F. A. Moritz
    • Institut für BiologieMartin-Luther-Universität Halle-Wittenberg
    • Department of Zoology and EntomologyUniversity of Pretoria
Original article

DOI: 10.1007/s13592-011-0092-y

Cite this article as:
Jarosch, A. & Moritz, R.F.A. Apidologie (2012) 43: 128. doi:10.1007/s13592-011-0092-y

Abstract

RNA interference involves the targeted knockdown of mRNA triggered by complementary dsRNA molecules applied to an experimental organism. Although this technique has been successfully used in honeybees (Apis mellifera), it remains unclear whether the application of dsRNA leads to unintended expression knockdown in unspecific, non-targeted genes. Therefore, we studied the gene expression of four non-target genes coding for proteins that are involved in different physiological processes after treatment with three dsRNAs in two abdominal tissues. We found unspecific gene downregulation depending on both the dsRNA used and the different tissues. Hence, RNAi experiments in the honeybee require rigid controls and carefully selected dsRNA sequences to avoid misinterpretation of RNAi-derived phenotypes.

Keywords

RNA interferencehoneybeesoff-target effectsreal-time PCR

Copyright information

© INRA, DIB-AGIB and Springer Science+Business Media B.V. 2011