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MicroRNA-98 inhibits TGF-β1-induced differentiation and collagen production of cardiac fibroblasts by targeting TGFBR1

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Abstract

To investigate the effects of miR-98 on TGF-β1-induced cardiac fibrosis in human cardiac fibroblasts (HCFs), and to establish the mechanism underlying these effects, HCFs were transfected with miR-98 inhibitor or mimic, and then treated with or without TGF-β1. The level of miR-98 was determined by qRT-PCR in TGF-β1-induced HCFs. Cell differentiation and collagen accumulation of HCFs were detected by qRT-PCR and Western blot assays, respectively. The mRNA and protein expressions of TGFBR1 were determined by qRT-PCR and Western blotting. In this study, the outcomes showed that TGF-β1 could dramatically decrease the level of miR-98 in a time- and concentration-dependent manner. Upregulation of miR-98 dramatically improved TGF-β1-induced increases in cell differentiation and collagen accumulation of HCFs. Moreover, bioinformatics analysis predicted that the TGFBR1 was a potential target gene of miR-98. Luciferase reporter assay demonstrated that miR-98 could directly target TGFBR1. Inhibition of TGFBR1 had the similar effect as miR-98 overexpression. Downregulation of TGFBR1 in HCFs transfected with miR-98 inhibitor partially reversed the protective effect of miR-98 overexpression on TGF-β1-induced cardiac fibrosis in HCFs. Upregulation of miR-98 ameliorates TGF-β1-induced differentiation and collagen accumulation of HCFs by downregulation of TGFBR1. These results provide further evidence for protective effect of miR-98 overexpression on TGF-β1-induced cardiac fibrosis.

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Authors and Affiliations

  1. Affiliated Hospital, Medical Department, Hebei University of Engineering, Handan, 056002, Hebei, People’s Republic of China

    Ranran Cheng & Yan Zhou

  2. Emergency Department, Affiliated Hospital, Hebei University of Engineering, Congtai Road No. 81, Handan, 056002, Hebei, People’s Republic of China

    Ruiying Dang

  3. College of Medicine, Hebei University of Engineering, Handan, 056002, Hebei, People’s Republic of China

    Ranran Cheng, Yan Zhou, Min Ding & Huikun Hua

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  1. Ranran Cheng
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  2. Ruiying Dang
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  4. Min Ding
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Correspondence to Ranran Cheng.

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Cheng, R., Dang, R., Zhou, Y. et al. MicroRNA-98 inhibits TGF-β1-induced differentiation and collagen production of cardiac fibroblasts by targeting TGFBR1. Human Cell 30, 192–200 (2017). https://doi.org/10.1007/s13577-017-0163-0

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  • DOI: https://doi.org/10.1007/s13577-017-0163-0

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