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Agrobacterium T-DNA insertion in the rice DWARF SHOOT AND DEFECTIVE PANICLE1 (DSDP1) gene causes a severe dwarf phenotype, reduces plant vigour, and affects seed germination

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Abstract

We report a new T-DNA-tagged rice plant chi7, which displays a severe dwarf phenotype, reduced plant vigour, and impaired panicle development in the homozygous state. By chromosome walking, T-DNA integration was mapped in chromosome 2, 1054-bp upstream of the translation start site of a gene (Os02g0820400), which we designate as DWARF SHOOT AND DEFECTIVE PANICLE1 (DSDP1). DSDP1 expression was unexpectedly higher in the homozygous mutant leaves than in the hemizygous mutant and control plant leaves. Mutant dsdp1 seeds, stored for 24 weeks, failed to germinate in soil. The growth vigour of the dsdp1 mutant reduced with increasing seed storage period. The dsdp1 mutant plants, grown in vitro on MS medium, formed short, stout, and ageotropic roots with lesser number of root hairs. The findings suggest that DSDP1 may function as a negative regulator of many developmental processes in rice.

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Abbreviations

BR:

Brassinosteroid

GA:

Gibberellic acid

HygR :

Hygromycin resistant

IDVs:

Integrated density values

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Acknowledgements

We thank Dr. K. Dharmalingam, School of Biotechnology, Madurai Kamaraj University for his permission to use the radioisotope facility. This work was funded by the Department of Biotechnology, Ministry of Science and Technology, Government of India [Project entitled “Functional Analysis of Gene Regulatory Networks during Flower and Seed development in rice”, Project No. BT/AB/FG-I(PH-II)(5)2009]. University Grants Commission, Govt. India is acknowledged for Faculty Fellowship to K.V. [No. F. 18-1/2011 (BSR)].

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Correspondence to Karuppannan Veluthambi.

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Debjani Basu and Bharat Bhusan Majhi have contributed equally to this work.

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Basu, D., Majhi, B.B., Sripriya, R. et al. Agrobacterium T-DNA insertion in the rice DWARF SHOOT AND DEFECTIVE PANICLE1 (DSDP1) gene causes a severe dwarf phenotype, reduces plant vigour, and affects seed germination. J. Plant Biochem. Biotechnol. 26, 395–405 (2017). https://doi.org/10.1007/s13562-017-0400-3

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