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A polyphenolic compound rottlerin demonstrates significant in vitro cytotoxicity against human cancer cell lines: isolation and characterization from the fruits of Mallotus philippinensis

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Abstract

In vitro assay for cytotoxic activity of glands/hairs obtained from the fruits of Mallotus philippinensis has been carried out against 14 human cancer cell lines from nine different origins via 95% ethanolic, 50% ethanolic and aqueous extract at the concentration of 100 μg/ml. Results revealed that the 95% ethanolic extract showed highest in vitro cytotoxic effect against all the 14 human cancer cell lines. The fractions of the same extract i.e. 95% ethanolic were obtained and it was found that the significant cytotoxic potential was produced by the chloroform soluble fraction at 100 μg/ml as this fraction inhibited the growth of ten human cancer cell lines from seven different tissues. Further, the chromatographic analysis of the said fraction afforded a polyphenolic molecule rottlerin. This drug at the concentration of 1 × 10−5M and 1 × 10−4M suppressed the proliferation of eight human cancer cell lines from six different tissues and proved its exceptionally remarkable in vitro anticancer efficiency.

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Abbreviations

DMEM:

Dulbecco’s minimum essential medium

NCCS:

National Centre for Cell Science

NCI:

National Cancer Institute

PBS:

Phosphate buffer saline

RPMI:

Rosewell Park Memorial Institute

SRB:

Sulphorhodamine blue

TLC:

Thin layer chromatography

References

  • Ahluwalia VK, Sharma ND, Mittal B (Late), Gupta SR (1988) Novel prenylated flavanoids from Mallotus philippinensis Muell. Arg Ind J Chem 22B: 238–241

  • Anderson A (1885) Kamala resin – rottlerin. Edin New Phil Journ 1:296–300

    Google Scholar 

  • Chan FL, Choi HL, Chen ZY, Chan PS, Huang Y (2000) Induction of apoptosis in prostate cancer cell lines by a flavonoid. Cancer Lett 160:219–228

    Article  PubMed  CAS  Google Scholar 

  • Gresshoff M (1898) Tweete vervolg van het Onderzoek naar de Plantenstoffen van Nederlandsch Indie. Batavia gr 8 Cart M 6: 173–176

    Google Scholar 

  • Gupta SC, Sharma VN, Agarwaal JS (1952) Chemical constitution of the seeds of Mallotus philippinensis. Jour Sci Ind Res 11B:463–468

    CAS  Google Scholar 

  • Gujral ML, Varma DR, Sareen KN (1960a) Oral contraceptives (PartI) Antifertility effect of Mallotus philippinensis. Ind Jour Med Res 48:46–51

    CAS  Google Scholar 

  • Gujral ML, Varma DR, Sareen KN, Roy AK (1960b) Oral contraceptives (PartII) antifertility effect of Mallotus philippinensis. Ind Jour Med Res 48:52–58

    CAS  Google Scholar 

  • Hirano T, Gotoh M, Oka K (1994) Natural flavonoids and lignans are potent cytostatic agents against human leukemic HL-60 cells. Life Sci 55:1061–1069

    Article  PubMed  CAS  Google Scholar 

  • Khorana ML, Motiwala DK (1949) Anthelmintic activity of Kamala and its constituents. Ind Jour Pharm 11:37–43

    CAS  Google Scholar 

  • Monks A, Scudiero D, Skehan P, Shoemaker R, Paull K, Vistica D, Hose C, Langley J, Cronisc T, Vaigro-Wolff A, Gray-Goodrich M, Campbell H, Maya J, Boyd M (1991) Feasibility of a high—flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst 83:757–766

    Article  PubMed  CAS  Google Scholar 

  • Nagamachi A (1921) Uber die pharmakologisch Wirkung des Rottlerins des Hauptbestandteils der Kamala: Acta scholae med univ imp. Bioto 4:307–317

    CAS  Google Scholar 

  • Perkin AG, Perkin WH (1886) Notiz iiber kamala – Mallotoxin. Ber 19:3109–3110

    Google Scholar 

  • Robertson A (1937) Rottlerin Part I. Jour Chem Soc 1:748–750

    Google Scholar 

  • Sasidharan N (1987) Forest trees of Kerala. Forest Research Institute, Peechi

    Google Scholar 

  • Satyavati GV, Gupta KAA, Tandon N (eds.) (1987) Medicinal plants of India, ICMR 2: 201–210

  • Skehan P, Storeng R, Scudiero D, Monks A, McMohan J, Vistica D, Warren JT, Bokesh H, Kenny S, Boyd M (1990) New colorimetric cytotoxcity assay for anticancer drug screening. J Natl Cancer Inst 82:1107–1112

    Article  PubMed  CAS  Google Scholar 

  • Tanaka T, Ito T, Iinuma M, Takahashi Y, Naganawa H (1998) Dimeric chalcone derivatives from Mallotus philippinensis. Phytochemistry 48:1423–1427

    Article  CAS  Google Scholar 

  • The Wealth of India (1962) Dictionary of Indian Raw Materials and Industrial Products CSIR: New Delhi 5: 229–240

  • Widen CJ, Puri HS (1980) Natural occurance and chemical variability of phloroglucinols in Kamala. Planta Medica 40:284–287

    Article  CAS  Google Scholar 

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Acknowledgments

The author is thankful to Indian Institute of Integrative Medicine (CSIR), Jammu for providing technical support.

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Authors and Affiliations

  1. Division of Biochemistry and Plant Physiology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha, Jammu, 180009 (J&K), India

    Vikas Sharma

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  1. Vikas Sharma
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Correspondence to Vikas Sharma.

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Sharma, V. A polyphenolic compound rottlerin demonstrates significant in vitro cytotoxicity against human cancer cell lines: isolation and characterization from the fruits of Mallotus philippinensis . J. Plant Biochem. Biotechnol. 20, 190–195 (2011). https://doi.org/10.1007/s13562-011-0045-6

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  • DOI: https://doi.org/10.1007/s13562-011-0045-6

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