Journal of NeuroVirology

, Volume 22, Issue 5, pp 555–563

Overexpression of thyroid hormone receptor β1 altered thyroid hormone-mediated regulation of herpes simplex virus-1 replication in differentiated cells

  • Feng Chen
  • Robert W. Figliozzi
  • Gautam Bedadala
  • Jayavardhana Palem
  • S. Victor Hsia
Article

DOI: 10.1007/s13365-016-0423-x

Cite this article as:
Chen, F., Figliozzi, R.W., Bedadala, G. et al. J. Neurovirol. (2016) 22: 555. doi:10.1007/s13365-016-0423-x

Abstract

Thyroid hormone (T3) has been suggested to play a role in herpes simplex virus 1 (HSV-1) replication. It was previously reported that HSV-1 replication was suppressed by T3 in mouse neuroblastoma cells overexpressing thyroid hormone receptor β1 (TRβ1). Using a human neuro-endocrine cells LNCaP differentiated by androgen deprivation, HSV-1 replication was active but decreased by T3 at very low moi, probably due to low copy of TRβ1. In this study, a recombinant HSV-1 was constructed expressing TRβ1 (HSV-1/TRβ1). Infection of Vero cells (very little TRβ1 expression) with HSV-1/TRβ1 exhibited increased replication in the presence of T3 compared to the counterpart without TRβ1 overexpression. Interestingly, HSV-1/TRβ1 infection of differentiated LNCaP cells showed strong suppression of viral replication by T3 and the removal of hormone did not fully reversed the suppression as was observed in parent virus. Quantitative analyses indicated that ICP0 expression was blocked using HSV-1/TRβ1 for infection during T3 washout, suggesting that overexpression of TRβ1 is likely to delay its inhibitory effect on viral gene expression. Together these results emphasized the importance of TRβ1 in the regulation of HSV-1 replication in differentiated environment with neuronal phenotype.

Keywords

Herpes simplex virusThyroid hormoneDifferentiationNeuronsPlaque assayThyroid hormone receptor beta 1

Abbreviations

HSV-1

Herpes simplex virus type-1

T3

Thyroid hormone

qPCR

Quantitative polymerase chain reaction

qRT-PCR

Quantitative reverse transcription polymerase chain reaction

moi

Multiplicity of infection

GFP

Green fluorescent protein

DAPI

4′,6-Diamidino-2-phenylindole

PPIA

Peptidylprolyl isomerase A

dpi

Days post infection

CHX

Cycloheximide

TRβ1

Thyroid hormone receptor beta 1

TRE

Thyroid hormone receptor element

ACV

Acyclovir

Copyright information

© Journal of NeuroVirology, Inc. 2016

Authors and Affiliations

  • Feng Chen
    • 1
  • Robert W. Figliozzi
    • 1
    • 2
  • Gautam Bedadala
    • 1
    • 3
  • Jayavardhana Palem
    • 3
  • S. Victor Hsia
    • 1
    • 2
  1. 1.Department of Pharmaceutical Sciences, School of Pharmacy and Health ProfessionsUniversity of Maryland Eastern ShorePrincess AnneUSA
  2. 2.Department of Natural Sciences, School of Agriculture and Natural SciencesUniversity of Maryland Eastern ShorePrincess AnneUSA
  3. 3.Department of Basic Pharmaceutical Sciences, College of PharmacyUniversity of Louisiana MonroeMonroeUSA