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Perivascular inflammatory cells in ovine Visna/maedi encephalitis and their possible role in virus infection and lesion progression

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Abstract

We examined the distribution in the perivascular spaces of Visna/maedi antigen, T cells (CD3+, CD4+ and CD8+), B cells and macrophages by immunohistochemistry in 22 natural cases of Visna/maedi encephalitis. Sheep showed lymphocytic or histiocytic lesions. In mild lymphocytic lesions, the viral antigen was detected in perivascular cuffs where CD8+ T cells predominated, but in severe lymphocytic lesions, sparse antigen was identified, and CD8+/CD4+ T cells appeared in a similar proportion in multilayer perivascular sleeves. In histiocytic lesions, vessels were surrounded by macrophages with abundant viral antigen, with CD8+/CD4+ T cells and B cells in the periphery. These results could reflect different stages of virus neuroinvasion and clarify the neuropathogenesis of Visna/maedi encephalitis.

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Acknowledgments

This work was supported by grants from the Spanish Ministry of Education (AGL2007-66874-CO4-04/GAN) and from the Junta of Castilla y León (LE36A12-1). L. Polledo holds a grant from the Ministry of Education (AP2007-02007). We thank the veterinarians for providing cases and Gloria Belver for technical assistance.

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Authors and Affiliations

  1. Pathological Anatomy Section, Animal Health Department, Veterinary School, University of León, 24007, León, Spain

    Laura Polledo, Jorge González, Julio Benavides, Beatriz Martínez-Fernández, Ma. Carmen Ferreras & Juan F. García Marín

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  1. Laura Polledo
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  2. Jorge González
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  3. Julio Benavides
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  4. Beatriz Martínez-Fernández
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  5. Ma. Carmen Ferreras
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  6. Juan F. García Marín
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Correspondence to Laura Polledo.

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Polledo, L., González, J., Benavides, J. et al. Perivascular inflammatory cells in ovine Visna/maedi encephalitis and their possible role in virus infection and lesion progression. J. Neurovirol. 18, 532–537 (2012). https://doi.org/10.1007/s13365-012-0131-0

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  • DOI: https://doi.org/10.1007/s13365-012-0131-0

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