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Multiphoton Dissociation of Electrosprayed MegaDalton-Sized DNA Ions in a Charge-Detection Mass Spectrometer

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Journal of The American Society for Mass Spectrometry

Abstract

Charge detection mass spectrometry in combination with a linear electrostatic ion trap coupled to a continuous wavelength infrared CO2 laser has been used to study the multiphoton dissociation of DNA macromolecular ions. Samples, with masses ranging from 2.23 to 31.5 MDa, include single strand circular M13mp18, double strand circular M13mp18, and double strand linear LambdaPhage DNA fragments. Their activation energies for unimolecular dissociation were determined. Activation energy values slightly increase as a function of the molecular weight. The most important result is the difference between the fragmentations observed for hybridized double-strands and dimers of single strands.

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Acknowledgments

The authors gratefully acknowledge the ANR for financial support of this work (grants no. ANR-08 BLAN-0110-01 and no. ANR-11-PDOC-032-01). The authors thank Xavier Dagany, Christian Clavier, Michel Kerleroux, Marc Barbaire, Jacques Maurelli, and Congyu Bao for their invaluable assistance.

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Authors and Affiliations

  1. Université Claude Bernard Lyon1-CNRS, Université de Lyon, 69622, Villeurbanne cedex, France

    Tristan Doussineau, Pierre Paletto, Philippe Dugourd & Rodolphe Antoine

  2. Institut lumière matière, UMR5306, Villeurbanne, France

    Tristan Doussineau, Pierre Paletto, Philippe Dugourd & Rodolphe Antoine

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  1. Tristan Doussineau
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  2. Pierre Paletto
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  3. Philippe Dugourd
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  4. Rodolphe Antoine
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Correspondence to Rodolphe Antoine.

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Doussineau, T., Paletto, P., Dugourd, P. et al. Multiphoton Dissociation of Electrosprayed MegaDalton-Sized DNA Ions in a Charge-Detection Mass Spectrometer. J. Am. Soc. Mass Spectrom. 26, 7–13 (2015). https://doi.org/10.1007/s13361-014-1011-z

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  • DOI: https://doi.org/10.1007/s13361-014-1011-z

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