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Development and characterization of microsatellite markers for the fruit fly, Bactrocera tau (Diptera: Tephritidae)

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Abstract

The fruit fly, Bactrocera tau (Walker), is one of the most important invasive worldwide pests on fruits and vegetables. In order to facilitate species identification and population genetic analysis, we isolated 22 microsatellite loci through an enriched genomic library protocol. Polymorphisms were evaluated in 30 individuals. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosities ranged from 0.048 to 0.862 and 0.136 to 0.852, respectively. The polymorphic information content values were from 0.124 to 0.818. Eight loci had significant deviations from the Hardy–Weinberg equilibrium. These SSR markers can be used to understand the population genetics and evolutionary patterns of the B. tau complex.

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Acknowledgments

This work was supported by the Key Program for Science and Technology and the Major Projects of Hainan, China (ZDXM20130049 and ZDZX2013008), and the Scientific Research Project of CATAS (1630042014002).

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Correspondence to Weiquan Qin.

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Yan, W., Liu, L., Huang, S. et al. Development and characterization of microsatellite markers for the fruit fly, Bactrocera tau (Diptera: Tephritidae). Appl Entomol Zool 50, 545–548 (2015). https://doi.org/10.1007/s13355-015-0359-2

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  • DOI: https://doi.org/10.1007/s13355-015-0359-2

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