Abstract
The carcasses of yellow-fat rabbits may be attractive to modern consumers, because they have a relatively high content of biologically active compounds. One of the main candidate genes associated with the yellow-fat trait is β-carotene 9′,10′-oxygenase (BCO2). This study is the first report of the novel AAT-deletion mutation at codon 248 of the BCO2 gene, which has been found in homozygous yellow-fat rabbits. The deletion mutation, located at the beginning of exon 6, results in the absence of asparagine in protein. We also developed a PCR-RFLP test that supports intravital genotyping of indel polymorphism based on genomic DNA.
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References
Amengual J, Lobo GP, Golczak M, Li HN, Klimova T, Hoppel CL, Wyss A, Palczewski K, von Lintig J (2011) A mitochondrial enzyme degrades carotenoids and protects against oxidative stress. FASEB J 25:948–959
Gugołek A, Antoszkiewicz Z, Daszkiewicz T, Cilulko J, (2010) Characteristics of visceral fat in rabbits and pigeons. Proc IV Int Sci Conf., Meat in technology and human nutrition, Poznań-Rosnówko, pp. 81–82.
Kotake-Nara E, Nagao A (2011) Absorption and metabolism of xantophylls. Mar Drugs 9:1024–1037
Lietz G, Oxley A, Boesch-Saadatmandi C, Kobayashi D (2012) Importance of β, β-carotene 15,15′-monooxygenase 1 (BCMO1) and β, β-carotene 9′,10′-dioxygenase 2 (BCDO2) in nutrition and health. Mol Nutr Food Res 56:241–250
Lindqvist A, He Y-G, Andersson S (2005) Cell type-specific expression of β-carotene 9′,10′-monooxygenase in human tissues. J Histochem Cytochem 53:1403–1412
Moise AR, von Lintig J, Palczewski K (2005) Related enzymes solve evolutionarily recurrent problems in the metabolism of carotenoids. Trends Plant Sci 10:178–186
Pease M (1930) The inheritance of yellow-fat in rabbits. Verh 1 Internat Kaninchenzuchter Kongresses, Leipzig pp. 91–95.
Tian R, Pitchford WS, Morris CA, Cullen NG, Bottema CDK (2009) Genetic variation in the β, β-carotene 9′, 10′-dioxygenase gene and association with fat colour in bovine adipose tissue and milk. Anim Genet 41:253–259
Tian R, Cullen NG, Morris CA, Fisher PJ, Pitchford WS, Bottema CDK (2012) Major effect of retinal short-chain dehydrogenase reductase (RDHE2) on bovine fat colour. Mamm Genome 23:378–386
Våge DI, Boman IA (2010) A nonsense mutation in the beta-carotene oxygenase 2 (BCO2) gene is tightly associated with accumulation of carotenoids in adipose tissue in sheep (Ovis aries). BMC Genet 11:10
Wilson WK, Dudley FJ (1946) Fat colour and fur colour in different varieties of rabbit. J Genet 47(3):290–294
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Communicated by: Maciej Szydlowski
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Strychalski, J., Brym, P., Czarnik, U. et al. A novel AAT-deletion mutation in the coding sequence of the BCO2 gene in yellow-fat rabbits. J Appl Genetics 56, 535–537 (2015). https://doi.org/10.1007/s13353-015-0290-9
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DOI: https://doi.org/10.1007/s13353-015-0290-9