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Genome-wide association mapping of preharvest sprouting resistance in a diversity panel of European winter wheats

  • Plant Genetics • Original Paper
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Abstract

Global wheat production will benefit from cultivars showing genetic resistance to preharvest sprouting (PHS). Working on PHS resistance is still challenging due to the lack of simple protocols for the provocation of symptoms for appropriate trait differentiation under highly variable environmental conditions. Therefore, the availability of molecular markers for enhancing PHS resistance in breeding lines is of utmost importance. Genome-wide association mapping was performed to unravel the genetics of PHS resistance in a diversity panel of 124 winter wheat genotypes using both random and targeted marker locus approaches. Data for grain germination tests, spike wetting treatments, and field sprouting damage measurements of grains were collected in 11, 12, and four environments, respectively. Twenty-two quantitative trait loci (QTL) linked with 40 markers were detected for the three traits commonly used for assessing the PHS resistance of cultivars. All but five QTL on chromosomes 1B, 1D (two QTL), 3D, and 5D showed locations similar to previous studies, including prominent QTL on chromosomes 2BS, 3AS, and 4AL. The highest retrieval rate across environments was found for QTL on chromosomes 1D, 2BS, 3D, 4AL, and 7B. The study identified genomic signatures useful for marker-assisted improvement of PHS resistance not only in European breeding programs, but of global significance.

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Acknowledgments

Excellent technical assistance from Petra Greim and Josef Schuhbauer from LfL and Manuela Dinterer and Thomas Miksch from AGES is highly appreciated. We also thank all people at LfL, AGES, and breeding companies who assisted in fulfilling the research aims. The international ERANET-CORNET research project named “Efficient phenotypic and molecular selection methods for enhancing preharvest sprouting tolerance of European wheat germplasm”—Robust Wheat (CORNET 825860) was funded by the FFG (Austrian Research Promotion Agency), the Austrian Seed Association, and the Börse für landwirtschaftliche Produkte in Wien. German Funding: “Das IGF-Vorhaben 39 EN/1 der Forschungsvereinigung Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung e.V.—GFP, Kaufmannstraße 71, 53115 Bonn wurde über die AiF im Rahmen des Programms zur Förderung der Industriellen Gemeinschaftsforschung und -entwicklung (IGF) vom Bundesministerium für Wirtschaft und Technologie aufgrund eines Beschlusses des Deutschen Bundestages gefördert”.

Conflict of interest

The authors declare that they have no conflict of interest.

Compliance with ethical standards

All experiments included in this study comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. Institute for Crop Science and Plant Breeding, Bavarian State Research Center for Agriculture (LfL), Am Gereuth 8, 85354, Freising, Germany

    Theresa Albrecht, Lorenz Hartl & Volker Mohler

  2. Austrian Agency for Health and Food Safety (AGES), Spargelfeldstraße 191, 1220, Vienna, Austria

    Michael Oberforster

  3. Secobra Saatzucht GmbH, Feldkirchen 3, 85368, Moosburg, Germany

    Hubert Kempf

  4. Saatzucht Josef Breun GmbH & Co. KG, Amselweg 1, 91074, Herzogenaurach, Germany

    Ludwig Ramgraber

  5. Limagrain GmbH, Salder Straße 4, 31226, Peine-Rosenthal, Germany

    Johannes Schacht

  6. Lantmännen SW Seed Hadmersleben GmbH, Kroppenstedter Straße 4, 39398, Hadmersleben, Germany

    Ebrahim Kazman

  7. Saatzucht LFS Edelhof, Edelhof 1, 3910, Zwettl, Austria

    Elisabeth Zechner

  8. Saatzucht Donau GesmbH & CoKG, Reichersberg 86, 4981, Reichersberg am Inn, Austria

    Anton Neumayer

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  1. Theresa Albrecht
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  2. Michael Oberforster
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  3. Hubert Kempf
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  4. Ludwig Ramgraber
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  5. Johannes Schacht
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  6. Ebrahim Kazman
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  7. Elisabeth Zechner
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  8. Anton Neumayer
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  9. Lorenz Hartl
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  10. Volker Mohler
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Correspondence to Volker Mohler.

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Communicated by: Andrzej Górny

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Albrecht, T., Oberforster, M., Kempf, H. et al. Genome-wide association mapping of preharvest sprouting resistance in a diversity panel of European winter wheats. J Appl Genetics 56, 277–285 (2015). https://doi.org/10.1007/s13353-015-0286-5

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  • DOI: https://doi.org/10.1007/s13353-015-0286-5

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