May 2012, Volume 53, Issue 2, pp 237-247,
Open Access This content is freely available online to anyone, anywhere at any time.
Date: 25 Feb 2012
Genetic variation of Fusarium oxysporum isolates forming fumonisin B1 and moniliformin
Thirty single-spore isolates of a toxigenic fungus, Fusarium oxysporum, were isolated from asparagus spears and identified by species-specific polymerase chain reaction (PCR) and translation elongation factor 1-α (TEF) sequence analysis. In the examined sets of F. oxysporum isolates, the DNA sequences of mating type genes (MAT) were identified. The distribution of MAT idiomorph may suggest that MAT1-2 is a predominant mating type in the F. oxysporum population. F. oxysporum is mainly recognised as a producer of moniliformin—the highly toxic secondary metabolite. Moniliformin content was determined by high-performance liquid chromatography (HPLC) analysis in the range 0.05–1,007.47 μg g−1 (mean 115.93 μg g−1) but, also, fumonisin B1 was detected, in the concentration range 0.01–0.91 μg g−1 (mean 0.19 μg g−1). There was no association between mating types and the mycotoxins biosynthesis level. Additionally, a significant intra-species genetic diversity was revealed and molecular markers associated with toxins biosynthesis were identified.
- Genetic variation of Fusarium oxysporum isolates forming fumonisin B1 and moniliformin
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
Journal of Applied Genetics
Volume 53, Issue 2 , pp 237-247
- Cover Date
- Print ISSN
- Online ISSN
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- Fusarium oxysporum
- Fumonisin B1
- Mating type idiomorphs
- Genetic fingerprinting
- HPLC analysis
- Author Affiliations
- 1. Department of Phytopathology, Poznan University of Life Sciences, Dąbrowskiego 159, 60-594, Poznań, Poland
- 2. Department of Mathematical and Statistical Methods, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637, Poznan, Poland
- 3. Department of Chemistry, Poznan University of Life Sciences, Wojska Polskiego 75, 60-625, Poznań, Poland
- 4. Institute of Plant Genetics Polish Academy of Science, Strzeszyńska 34, 60-479, Poznań, Poland