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In vivo comparison of biomineralized scaffold-directed osteogenic differentiation of human embryonic and mesenchymal stem cells

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Abstract

Human pluripotent stem cells such as embryonic stem cells (hESCs) and multipotent stem cells like mesenchymal stem cells (hMSCs) hold great promise as potential cell sources for bone tissue regeneration. Comparing the in vivo osteogenesis of hESCs and hMSCs by biomaterial-based cues provides insight into the differentiation kinetics of these cells as well as their potential to contribute to bone tissue repair in vivo. Here, we compared in vivo osteogenic differentiation of hESCs and hMSCs within osteoinductive calcium phosphate (CaP)-bearing biomineralized scaffolds that recapitulate a bone-specific mineral microenvironment. Both hESCs and hMSCs underwent osteogenic differentiation responding to the biomaterial-based instructive cues. Furthermore, hMSCs underwent earlier in vivo osteogenesis compared to hESCs, but both stem cell types acquired a similar osteogenic maturation by 8 weeks of implantation.

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Acknowledgments

This work was supported by National Institutes of Health (NIH; Grant 1 R01 AR063184-01A1). The hMSCs used in this study were provided by Texas A&M University from the NIH (Grant P40RR017447).

Conflict of interest

Authors declare no conflict of interest.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Southeast University, Sipailou 2#, Nanjing, Jiangsu Province, 210096, People’s Republic of China

    Cai Wen

  2. Department of Bioengineering, University of California— San Diego, 9500, Gilman Drive, La Jolla, CA, 92093-0412, USA

    Heemin Kang, Yu-Ru V. Shih, YongSung Hwang & Shyni Varghese

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  1. Cai Wen
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  2. Heemin Kang
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  3. Yu-Ru V. Shih
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  4. YongSung Hwang
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  5. Shyni Varghese
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Correspondence to Shyni Varghese.

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Wen, C., Kang, H., Shih, YR.V. et al. In vivo comparison of biomineralized scaffold-directed osteogenic differentiation of human embryonic and mesenchymal stem cells. Drug Deliv. and Transl. Res. 6, 121–131 (2016). https://doi.org/10.1007/s13346-015-0242-2

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