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Marker assisted introgression of QTL region to improve late leaf spot and rust resistance in elite and popular variety of groundnut (Arachis hypogaea L.) cv TMV 2

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Abstract

Late leaf spot (LLS) and rust diseases are the major devastating diseases in groundnut that lowers crop yield and fodder quality. Marker assisted backcross breeding was employed to introgress LLS and rust diseases resistance QTL region from GPBD 4 into an India’s popular groundnut variety TMV 2. The MABC approach was exercised using a total of eight disease resistance-linked markers viz.., IPAHM103, GM1573, GM1009, Seq8D09, GM2009, GM1536, GM2301 and GM2079 present in the QTL region. After three backcrosses and selfing, a total of 155 BC3F2 lines were developed from the cross TMV 2 x GPBD 4. Ten BC3F2 homozygous introgression lines were identified with resistance QTL region and subjected to background analysis. A genome recovery upto 99.80% was observed among introgression lines and advanced to further generations for field evaluation. Finally, six ILs with enhanced late leaf spot and rust diseases resistance were identified. Those ILs had significantly increased pod yield (42–65%) over the recurrent parent TMV 2 with enhanced resistance to LLS and rust diseases. Multi-location field evaluation of these ILs could leads to the release of improved varieties and also can be used as genetic resources in the groundnut improvement.

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References

  • Anderson WF, Wynne JC, Green CC (1986) Potential for incorporation of early and late leaf spot resistance in peanut. Plant Breed 97:163–170

    Article  Google Scholar 

  • Balakrishnan D, Robin S, Rabindran R, Senthil N, Raveendran M, John Joel A (2014) Marker assisted backcross breeding approach to improve blast resistance in Indian rice (Oryza sativa) variety ADT43. Euphytica. https://doi.org/10.1007/s10681-014-1146-9

  • Bromfield K, Bailey W (1972) Inheritance of resistance to Puccinia arachidis in peanut. Phytopathology 62:748

    Google Scholar 

  • Cuc LM, Mace ES, Crouch JH, Quang VD, Long TD, Varshney RK (2008) Isolation and characterization of novel microsatellite markers and their application for diversity assessment in cultivated groundnut (Arachis hypogaea). BMC Plant Biol 8:55–65

    Article  Google Scholar 

  • Dwivedi SL, Pande S, Rao JN, Nigam SN (2002) Components of resistance to late leaf spot and rust among interspecific derivatives and their significance in a foliar disease resistance breeding in groundnut (Arachis hypogaea L.). Euphytica 125:81–88

    Article  CAS  Google Scholar 

  • Gautami B, Fonce D, Pandey MK, Moretzsohn MC, Sujay V, Qin H, Hong Y, Faye I, Chen X, Prakash AB, Shah TM, Gowda MVC, Nigam SN, Liang X, Hoisington DA, Guo B, Bertioli DJ, Rami JF, Varshney RK (2012) An International reference consensus genetic map with 897 marker loci based on 11 mapping populations for tetraploid groundnut (Arachis hypogaea L.). PLoS One 7(7):1–11

  • Gowda MVC, Motagi BN, Naidu GK, Diddimani SB, Sheshagiri R (2002) GPBD 4: a Spanish bunch groundnut genotype resistant to rust and late leaf spot. Int Arachis Newsl 22:29–32

    Google Scholar 

  • Green CC, Wynne JC (1986) Diallel and generation means analyses for the components of resistance to Cercospora arachidicola in peanut. Theor Appl Genet 73:228–235

    Article  CAS  Google Scholar 

  • Green CC, Wynne JC (1987) Genetic variability and heritability for resistance to early leaf spot in four crosses of Virginia-type peanut. Crop Science 27:18–21

  • Hamid MA, Isleib TG, Wynne JC, Green CC (1981) Combining ability analysis of Cercospora leaf spot resistance and agronomic traits in Arachis hypogaea L. Oleagineux 36:605–612

    Google Scholar 

  • Jogloy S, Wynne JC, Beute MK (1987) Inheritance of late leaf spot resistance and agronomic traits in peanut. Peanut Sci 14:86–90

    Article  Google Scholar 

  • Khedikar YP, Gowda MVC, Sarvamangala C, Patgar KV, Upadhyaya HD, Varshney RK (2010) A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resist-ance in groundnut (Arachis hypogaea L.). Theor Appl Genet 121:71–984

    Article  Google Scholar 

  • Kolekar RM, Sujay V, Shirasawa K, Sukruth M, Khedikar YP, Gowda MVC, Pandey MK, Varshney RK, Bhat RS (2016) QTL mapping for late leaf spot and rust resistance using an improved genetic map and extensive phenotypic data on a recombinant inbred line population in peanut (Arachis hypogaea L.). Euphytica DOI. https://doi.org/10.1007/s10681-016-1651-0

    Article  Google Scholar 

  • Kolekar RM, Sukruth M, Shirasawa K, Nadaf HL, Motagi BN, Lingaraju S, Patil PV, Bhat RS (2017) Marker-assisted backcrossing to develop foliar disease resistant genotypes in TMV 2 variety of peanut. (Arachis hypogaea L.). Plant Breed 2017:1–6

  • Kornegay JL, Beute MK, Wynne JC (1980) Inheritance of resistance to Cercospora arachidicola and Cercosporidium personatum in six Virginia-type peanut lines. Peanut Sci 7:4–9

    Article  Google Scholar 

  • Mace ES, Buhariwalla HK, Crouch JH (2003) A high-throughput DNA extraction protocol for tropical molecular breeding programs. Plant Mol Biol Rep 21:459–459

  • Motagi (2001) Genetic analysis of resistance to late leaf spot and rust vis-à-vis productivity in groundnut (Arachis hypogaea L.). Dissertation, University of Agriculture Sciences, Dharwad

  • Nevill DJ (1982) Inheritance of resistance to Cercosporidium personatum in groundnuts: a genetic model and its implications for selection. Oleagineux 37:355–362

    Google Scholar 

  • Paramasivam K, Jayasekhar M, Rajasekharan R, Veerabadhiran P (1990) Inheritance of rust resistance in groundnut (Arachis hypogaea L.). Madras Agric J 77:50–52

    Google Scholar 

  • Selvaraj MG, Manivannan N, Schubert AM, Ayers JL, Baring MR, Burow MD (2009) Identification of QTLs for pod and kernel traits in cultivated peanut by Bulked Segregant Analysis. Electron J Biotechnol 12(2):1–10

    Google Scholar 

  • Sharief Y, Rawlings JO, Gregory WC (1978) Estimates of leaf spot resistance in three interspecific hybrids of Arachis. Euphytica 27:741–751

    Article  Google Scholar 

  • Shoba D, Manivannan N, Vindiyavarman P, Nigam SN (2013) Identification of Quantitative Trait Loci (QTL) for late leaf spot disease resistance in groundnut (Arachis hypogaea L.). Legume Res 36(5):467–472

    Google Scholar 

  • Subbarao PV, Subrahmanyam P, Reddy PM (1990) A modified nine point disease scale for assessment of rust and late leaf spot of groundnut. In: Second international congress of French phyto-pathological society. 28–30 November 1990, Montpellier, France, p 25

  • Subrahmanyam W, McDonald, and Gibbons (1984) The influence of foliar diseases and their control by selective fungicides on a range of groundnut (Arachis hypogaea L.) genotypes. Ann Appl Biol 104:467–476

    Article  CAS  Google Scholar 

  • Sujay V, Gowda MVC, Pandey MK, Bhat RS, Khedikar YP, Nadaf HL, Gautami B, Sarvamangala C, Lingaraju S, Radhakrishan T, Knapp SJ, Varshney RK (2012) Quantitative trait locus analy-sis and construction of consensus genetic map for foliar dis-ease resistance based on two recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.). Mol Breed 32(2):773–788

    Article  Google Scholar 

  • Tegelstrom H (1992) Detection of mitochondrial DNA fragments. In: Hoelzel AR (ed) Molecular genetic analysis of populations: a practical approach. IRL Press, Oxford, pp 89–114

    Google Scholar 

  • Tiwari S, Ghewande M, Misra D (1984) Inheritance of resistance to rust and late leaf spot in groundnut (Arachis hypogaea L.). J Cytol Genet 19:97–101

    Google Scholar 

  • Varshney RK, Graner A, Sorrells ME (2005) Genomics assisted breeding for crop improvement. Trends Plant Sci 10:621–630

    Article  CAS  Google Scholar 

  • Varshney RK, Hoisington DA, Tyagi AK (2006) Advances in cereal genomics and applications in crop breeding. Trends Biotechnol 24:490–499

    Article  CAS  Google Scholar 

  • Varshney RK, Mohan SM, Gaur PM, Charmarthi SK, Singh VK, Srinivasan S, Swapna N, Sharma M, Singh S, Pande S (2014a) Marker-assisted backcrossing to introgress resistance to fusarium wilt race 1 and ascochyta blight in C 214, an elite cultivar of chickpea. Plant Genome 7(1):1–11

    Article  Google Scholar 

  • Varshney RK, Pandey MK, Janila P, Nigam SN, Sudini H, Gowda MVC, Sriswathi M, Radhakrishnan T, Manohar SS, Nagesh P (2014b) Marker assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.). Theor Appl Genet. https://doi.org/10.1007/s00122-014-2338-3

  • Varshney RK, Thundi M, May GD, Jackson SA (2010) Legume genomics and breeding. Plant Breed Rev 33:257–304

    Google Scholar 

  • Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around the tm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Authors are thankful to DBT, New Delhi for the financial assistance provided to this study under the GOI scheme “Integrated MAS to develop groundnut varieties for resistance to foliar fungal diseases Rust and Late Leaf Spot”. We are grateful to UAS, Dharwad, Karnataka, India for sparing seeds of donor genotype GPBD 4 for this study.

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Authors and Affiliations

  1. Department of Oilseeds, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India

    P. Ramakrishnan, N. Manivannan, L. Mahaingam, R. Prabhu & P. Gopikrishnan

  2. National Pulses Research Centre, Tamil Nadu Agricultural University, Vamban, Pudukkottai - 622 303, Tamil Nadu, India

    P. Ramakrishnan & N. Manivannan

  3. Regional Research Station, Tamil Nadu Agricultural University, Vridhachalam - 606 001, Tamil Nadu, India

    A. Mothilal

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  1. P. Ramakrishnan
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  2. N. Manivannan
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  3. A. Mothilal
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  4. L. Mahaingam
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  5. R. Prabhu
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  6. P. Gopikrishnan
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Correspondence to P. Ramakrishnan.

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Ramakrishnan, P., Manivannan, N., Mothilal, A. et al. Marker assisted introgression of QTL region to improve late leaf spot and rust resistance in elite and popular variety of groundnut (Arachis hypogaea L.) cv TMV 2. Australasian Plant Pathol. 49, 505–513 (2020). https://doi.org/10.1007/s13313-020-00721-9

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