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The effects of p21 gene C98A polymorphism on development of uterine leiomyoma in southeast Iranian women

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Tumor Biology

Abstract

Uterine leiomyoma (UL) is a monoclonal tumor which arises from uninhibited proliferation of a single myometrial cell; therefore, the imbalance in cell cycle regulation could be a key event in its development. In the present study, we aimed to assess the association of p21 gene polymorphisms and UL. Genomic DNA was extracted from blood samples of 154 women with UL and 197 age-, BMI-, and ethnically matched controls. p21 C98A (rs1801270) and C70T (rs1059234) polymorphism genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The CA genotype of p21 C98A polymorphism was significantly higher in UL women (28 %) compared to the controls (18 %), and the UL risk was 1.8-fold greater in women with CA genotype compared to CC genotype before and after adjusting for age, BMI, and ethnicity (OR, 1.8 [95 % CI, 1.1 to 3]; P = 0.02). There was no association between the AA genotype of p21 C98A polymorphism and UL. Moreover, the frequency of p21 98A allele was significantly higher in the UL women compared to controls (17 vs. 12 %, p = 0.04). The p21 C70T polymorphism did not correlate with UL before and after adjusting for age, BMI, and ethnicity. There was no difference in haplotype frequency of p21 C70T and C98A polymorphisms between UL patients and the controls. CA genotype of p21 C98A polymorphism may be a risk factor for UL susceptibility; however, p21 C70T polymorphism did not associate with UL.

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Acknowledgment

This article was extracted from the MS thesis (registered number 2161) at Zahedan University of Medical Sciences. The authors would like to thank the Deputy of Research Affairs at the University for funding this project.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Saeedeh Salimi & Anoosh Naghavi

  2. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Saeedeh Salimi & Azam Hajizadeh

  3. Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran, Iran

    Minoo Yaghmaei

  4. Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Sodabeh Rezaie, Batool Teimoori, Mahboube Parache & Mojgan Mokhtari

  5. Department of Biostatistics and Epidemiology, School of Public Health, Zahedan University of Medical Sciences, Zahedan, Iran

    Mahnaz Shahrakypour

  6. Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Mahnaz Shahrakypour

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  1. Saeedeh Salimi
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  2. Azam Hajizadeh
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Correspondence to Minoo Yaghmaei.

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All participants were Iranian and gave their informed consent before participating in the study. The protocol of this study was approved by the Ethics Committee of Zahedan University of Medical Sciences and conducted in accordance with the Declaration of Helsiniki.

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Salimi, S., Hajizadeh, A., Yaghmaei, M. et al. The effects of p21 gene C98A polymorphism on development of uterine leiomyoma in southeast Iranian women. Tumor Biol. 37, 12497–12502 (2016). https://doi.org/10.1007/s13277-016-5078-y

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  • DOI: https://doi.org/10.1007/s13277-016-5078-y

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