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Resveratrol inhibits oral squamous cell carcinoma through induction of apoptosis and G2/M phase cell cycle arrest

  • Original Article
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Tumor Biology

Abstract

The present study was performed to investigate the effect of resveratrol (trans-3,4′,5-trihydroxystilbene) present as a natural phytoalexin in grapes, peanuts, and red wine on oral squamous cancer cell lines, SCC-VII, SCC-25, and YD-38. MTS assay and flow cytometry, respectively, were used for the analysis of inhibition of cell proliferation and apoptosis. Western blot analysis was performed to examine the effect of resveratrol on the expression of proteins associated with cell cycle regulation. The results revealed a concentration- and time-dependent inhibition of proliferation in all the three tested cell lines on treatment with resveratrol. The IC50 of resveratrol for SCC-VII, SCC-25, and YD-38 cell lines was found to be 0.5, 0.7, and 1.0 μg/ml, respectively, after 48-h treatment. Examination of the cell cycle analysis showed that resveratrol treatment induced cell cycle arrest in the G2/M phase and enhanced the expression of phospho-cdc2 (Tyr 15), cyclin A2, and cyclin B1 in the oral squamous cell carcinoma (OSCC) cells. It also caused a marked increase in the percentage of apoptotic cells as revealed by the fluorescence-activated cell sorting analysis. Thus, resveratrol exhibits inhibitory effect on the proliferation of OSCC oral cancer cells through the induction of apoptosis and G2/M phase cell cycle arrest.

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Conflicts of interest

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Authors’ contributions

Dong-Xu Liu and Xiao-Dong Yu designed the study. Dong-Xu Liu and Jing-lei Yang wrote the manuscript. Dong-Xu Liu and Jing-lei Yang performed out the experiments and the related statistical analysis. All authors approved the final version of the manuscript.

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    Authors and Affiliations

    1. School of Stomatology, Shandong University, No. 44-1, Wenhua Xi Road, Jinan, 250012, Shandong, China

      Xiao-Dong Yu & Dong-Xu Liu

    2. Department of Stomatology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, 250014, Shandong, China

      Xiao-Dong Yu & Wan-Lin Zhang

    3. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan, 250012, Shandong, China

      Xiao-Dong Yu & Dong-Xu Liu

    4. Department of Stomatology, Dongying People’s Hospital, Dongying, 257091, Shandong, China

      Jing-lei Yang

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    1. Xiao-Dong Yu
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    2. Jing-lei Yang
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    Correspondence to Dong-Xu Liu.

    Additional information

    Xiao-Dong Yu and Jing-lei Yang contributed equally to this work.

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    Yu, XD., Yang, Jl., Zhang, WL. et al. Resveratrol inhibits oral squamous cell carcinoma through induction of apoptosis and G2/M phase cell cycle arrest. Tumor Biol. 37, 2871–2877 (2016). https://doi.org/10.1007/s13277-015-3793-4

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    • DOI: https://doi.org/10.1007/s13277-015-3793-4

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