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Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma

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Tumor Biology

Abstract

Deregulation of microRNAs (miRNAs) is essential to tumor development, and serum miRNA profiles have been reported in several cancers. However, the serum miRNA profile in oral squamous cell carcinoma (OSCC) remained unclear. The present study aimed to explore abnormal miRNA profile in sera samples from OSCC patients and the association of miR-483-5p with patient prognosis. Microarray analysis was performed in sera from OSCC patients versus healthy controls. miR-483-5p expression was measured by reverse transcription (RT)-PCR and correlated to clinicopathological characteristics of OSCC patients. The prognostic significance was then evaluated with a Kaplan–Meier curve and log-rank tests, using a Cox proportional hazard model. According to microRNA array, 16 miRNAs were upregulated and 10 were downregulated in OSCC patient sera. miR-483-5p expression was significantly increased in OSCC patients (3.23-fold, p < 0.01), and this was significantly correlated with tumor nodal metastasis (TNM) stage and lymph nodal metastases (p < 0.01, p < 0.01). For predicting OSCC, receiver operating characteristic (ROC)/area under the curve (AUC) analysis confirmed a AUC of 0.85 (sensitivity of 0.853 and specificity of 0.746). OSCC patients with high serum miR-483-5p had lower survival than those with low expression, and multivariate analyses for overall survival revealed that high serum miR-483-5p expression was an independent prognostic factor for OSCC (HR = 2.32, 95 %CI 1.20–4.48). miR-483-5p expression increased in OSCC patient sera, and this may be a novel diagnostic and prognostic biomarker for OSCC.

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Acknowledgments

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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    Authors and Affiliations

    1. Department of Stomatology, Shihua General Hospital of Lanzhou, 733 West Fuli Road, Gansu, China

      Huanxi Xu & Ning Li

    2. Department of Endodontics, Lanzhou Stomatology Hospital, Gansu, China

      Yuqi Yang

    3. Department of Orthodontics, Lanzhou Stomatology Hospital, Gansu, China

      Hongmei Zhao

    4. Institute of Anatomy and Embryology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China

      Xuguang Yang

    5. Institute of Pathophysiology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China

      Yu Luo & Wei Liu

    6. Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China

      Yinxiang Ren

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    1. Huanxi Xu
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    2. Yuqi Yang
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    7. Wei Liu
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    8. Ning Li
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    Correspondence to Ning Li.

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    Huanxi Xu and Yuqi Yang contributed equally to this work.

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    Xu, H., Yang, Y., Zhao, H. et al. Serum miR-483-5p: a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma. Tumor Biol. 37, 447–453 (2016). https://doi.org/10.1007/s13277-015-3514-z

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    • DOI: https://doi.org/10.1007/s13277-015-3514-z

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