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Investigation of microRNA-155 as a serum diagnostic and prognostic biomarker for colorectal cancer

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Tumor Biology

Abstract

The aim of the present study was to explore serum microRNA-155 (miR-155) expression in patients with colorectal cancer (CRC) and examined the potential usefulness of this molecule as a biomarker for diagnosis and prognosis in CRC. Serum samples were obtained between May 2007 and March 2013 from 146 CRC patients and 60 healthy controls. Serum miR-155 expression levels were measured by quantitative real time reverse transcription-polymerase chain reaction (qRT-PCR). Survival curves were obtained using the Kaplan–Meier method and assessed by the log-rank test. The receiver operating characteristic (ROC) curve was used for the prediction of cut-off values of the markers. Serum miR-155 expression level on average was upregulated in CRC patients compared with the matched healthy controls (P < 0.001). ROC curve analysis showed that miR-155 was a useful marker for discriminating cases from healthy controls, with an area under the ROC curve (AUC) of 0.776 (95 % confidence interval (CI) 0.714 to 0.837, P < 0.001). Kaplan–Meier analysis with the log-rank test indicated that high serum miR-155 expression had a significant impact on overall survival (38.2 vs. 69.9 %; P < 0.001) and progression-free survival (34.8 vs. 66.0 %; P < 0.001). In conclusion, the detection of miR-155 levels in the serum might serve as a new tumor biomarker in the diagnosis and assessment of prognosis of CRC.

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Authors and Affiliations

  1. Department of Gastrointestinal Surgery, Yantai Yuhuangding Hospital, Yantai, 264000, Shandong, China

    Zhong-chuan Lv, Hong-bing Chen & Da-wei Zhao

  2. Department of General Surgery, Qixia People’s Hospital, Yantai, 265300, Shandong, China

    Yong-sheng Fan

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  1. Zhong-chuan Lv
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  2. Yong-sheng Fan
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  3. Hong-bing Chen
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  4. Da-wei Zhao
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Correspondence to Zhong-chuan Lv.

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Lv, Zc., Fan, Ys., Chen, Hb. et al. Investigation of microRNA-155 as a serum diagnostic and prognostic biomarker for colorectal cancer. Tumor Biol. 36, 1619–1625 (2015). https://doi.org/10.1007/s13277-014-2760-9

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  • DOI: https://doi.org/10.1007/s13277-014-2760-9

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