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Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a

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Tumor Biology

Abstract

Osteosarcoma is the most common type of bone cancer. In the present study, by way of PCR-based microarrays, we found that TUT1, a nucleotidyl transferase, was significantly downregulated in osteosarcoma, compared with adjacent normal tissues. In the current study, we performed PCR-based microarrays using the cDNA prepared from osteosarcoma and adjacent normal tissues. The enforced expression of TUT1 was able to inhibit cell proliferation in U2OS and MG63 cells, while its knockdown using small interfering RNA (siRNA) oligos promoted cell proliferation. At the molecular level, we found that TUT1 could inhibit the expression levels of PPARgamma and SREBP-1c, two key regulators in lipogenesis, through upregulation of microRNA-24 and microRNA-29a. Therefore, our results suggest that TUT1 may act as a tumor suppressor for osteosarcoma, which might provide a novel mechanism for the tumor development.

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Authors and Affiliations

  1. Department of Pharmacy, Shanghai Tongji Hospital, 200065, Shanghai, China

    De-qiu Zhu

  2. Department of Pharmacy, The Branch of the First People’s Hospital of Shanghai City (the fourth hospital), 200081, Shanghai, China

    Yue-fen Lou

  3. Department of Pharmacy, East Hospital, Tongji University School of Medicine, 200120, Shanghai, China

    Zhi-gao He

  4. Department of Pharmacy, Yangpu Hospital, Tongji University School of Medicine, 200090, Shanghai, China

    Min Ji

Authors
  1. De-qiu Zhu
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  2. Yue-fen Lou
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  3. Zhi-gao He
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  4. Min Ji
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Corresponding authors

Correspondence to Zhi-gao He or Min Ji.

Additional information

De-qiu Zhu and Yue-fen Lou contributed equally to this work.

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Zhu, Dq., Lou, Yf., He, Zg. et al. Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a. Tumor Biol. 35, 11829–11835 (2014). https://doi.org/10.1007/s13277-014-2395-x

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  • DOI: https://doi.org/10.1007/s13277-014-2395-x

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