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Association of functional polymorphism at the miR-502-binding site in the 3′ untranslated region of the SETD8 gene with risk of childhood acute lymphoblastic leukemia, a preliminary report

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Tumor Biology

Abstract

MicroRNAs (miRNAs), a class of non-coding RNAs, bind to the 3′ untranslated regions (UTRs) of mRNAs, where they interfere with translation of genes and are implicated in the pathogenesis of diverse diseases. In the present study, we evaluate the impact of rs16917496 polymorphism within the miR-502 miRNA seed region at the 3′UTR of SEDT8 on childhood acute lymphoblastic leukemia (ALL). This case-control study was done on 75 ALL and 115 healthy children. Genotyping of rs16917496 C/T polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that CT as well as CT + TT decreased the risk of ALL in comparison with CC genotype (odds ratio (OR) = 0.29, 95 % confidence intervals (95 % CI) = 0.11–0.78, P = 0.014 and OR = 0.31, 95 % CI = 0.12–0.82, P = 0.016, respectively). Our results demonstrated that SETD8 rs16917496 C/T polymorphism was associated with decreased risk of developing pediatric ALL in Zahedan, southeast Iran. Larger studies with different ethnicities are desired to validate our findings.

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Acknowledgments

This paper was based on MSc. thesis of MSN and Zahedan University of Medical Sciences, and University of Mazandaran provided the fund.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Mohammad Hashemi

  2. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Mohammad Hashemi

  3. Department of Cell and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

    Maryam Sheybani-Nasab & Fatemeh Roodbari

  4. Genetic of Non-communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Majid Naderi & Mohsen Taheri

Authors
  1. Mohammad Hashemi
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  2. Maryam Sheybani-Nasab
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  3. Majid Naderi
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  4. Fatemeh Roodbari
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  5. Mohsen Taheri
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Corresponding author

Correspondence to Mohammad Hashemi.

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Mohammad Hashemi and Maryam Sheybani-Nasab contributed equally to the preparation of the article.

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Hashemi, M., Sheybani-Nasab, M., Naderi, M. et al. Association of functional polymorphism at the miR-502-binding site in the 3′ untranslated region of the SETD8 gene with risk of childhood acute lymphoblastic leukemia, a preliminary report. Tumor Biol. 35, 10375–10379 (2014). https://doi.org/10.1007/s13277-014-2359-1

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  • DOI: https://doi.org/10.1007/s13277-014-2359-1

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