Tumor Biology

, Volume 34, Issue 4, pp 2459–2468

Altered expression of β-catenin, E-cadherin, and E-cadherin promoter methylation in epithelial ovarian carcinoma

  • Rahul Bhagat
  • C. S. Premalata
  • V. Shilpa
  • V. R. Pallavi
  • G. Ramesh
  • C. R. Vijay
  • Lakshmi Krishnamoorthy
Research Article

DOI: 10.1007/s13277-013-0797-9

Cite this article as:
Bhagat, R., Premalata, C.S., Shilpa, V. et al. Tumor Biol. (2013) 34: 2459. doi:10.1007/s13277-013-0797-9

Abstract

The aim of the study was to evaluate the immunoexpression of E-cadherin, β-catenin, and Ki-67, as well as the promoter methylation of E-cadherin gene in epithelial ovarian cancer (EOC), as well as to find a possible relationship between the immunoexpression and hypermethylation. Promoter methylation was studied using methylation-specific PCR in 86 malignant cases, 14 low malignant potential (LMP) tumors and 19 benign cystadenomas. Immunohistochemical expression was carried out in 64 malignant cases, 8 LMP tumors, and 11 benign cystadenomas. Immunoexpression of E-cadherin was reduced in EOC, while 100 % expression was seen in LMP tumors and benign cystadenomas. An interesting observation was the nuclear expression of E-cadherin in a high percentage of cancers, which showed a positive correlation with Ki-67. Β-Catenin expression showed heterogeneous localization with increased nuclear localization, which was significantly higher in cases that did not express E-cadherin. Promoter methylation of E-cadherin was 36, 14, and 11 % in EOC, LMP tumors, and benign cystadenomas, respectively. Our results suggest that reduced expression of E-cadherin is associated with promoter methylation of E-cadherin gene, in addition to providing evidence for the aberrant nuclear localization of E-cadherin in EOC.

Keywords

E-cadherinβ-CateninImmunohistochemistryPromoter methylationEOCLMP tumors

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Rahul Bhagat
    • 1
  • C. S. Premalata
    • 2
  • V. Shilpa
    • 1
  • V. R. Pallavi
    • 1
  • G. Ramesh
    • 1
  • C. R. Vijay
    • 3
  • Lakshmi Krishnamoorthy
    • 1
  1. 1.Department of BiochemistryKidwai Memorial Institute of OncologyBangaloreIndia
  2. 2.Department of PathologyKidwai Memorial Institute of OncologyBangaloreIndia
  3. 3.Department of Epidemiology & BiostatisticsKidwai Memorial Institute of OncologyBangaloreIndia