Abstract
spinster (spin) is a late endosome/lysosome membrane protein with the amino acid sequence of a lysosomal sugar carrier and expressed in the glial cells. Spin is required for autophagy and lysosome reformation by releasing lysosomal degradation products of autolysosome into the cytosol in Drosophila larvae and adults. However, such kind of function has not been investigated in embryos yet. In this study, for the first time, we examined the effects of spin mutation on the endocytic pathway and autophagy during embryogenesis. Loss-of-function spin mutation led to the abnormal process of early endosome/recycling endosome and the accumulation of enlarged autophagosome/autolysosome. These abnormal endocytic pathway and autophagy subsequently caused the malformation of head at embryonic stages. These results show that Spin is involved in the endocytic pathway and autophagy during embryogenesis as well as larval and adult stages.
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Acknowledgements
We are grateful to Dr. Gonzalez-Gaitan for providing anti-Rab antibodies. We also thank Dr. Davis for providing spin mutant fly stocks. We also thank Korea Basic Science Institute Chuncheon center for allowing us to use the confocal and scanning electron microscope. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (700-20100094).
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Ji-Sun Kim, Ki-Seok Park, Ok-kyu Park, Sang Hee Kim and Sang-Hak Jeon declare that they have no conflict of interest.
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The article does not contain any studies with human and this study was performed following institutional and national guidelines.
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Ji-Sun Kim and Ki-Seok Park have equally contributed to this work.
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Kim, JS., Park, KS., Park, Ok. et al. Novel phenotypes of Drosophila spinster locus on the head formation during embryogenesis. Genes Genom 39, 237–242 (2017). https://doi.org/10.1007/s13258-016-0513-4
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DOI: https://doi.org/10.1007/s13258-016-0513-4