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Detection of LINE RT elements in the olive flounder (Paralichthys olivaceus) genome and expression analysis after infection with S. parauberis

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Abstract

Long interspersed nuclear elements (LINEs) are widely distributed in the vertebrate genome, and can be either beneficial or detrimental to the host genome. Here we identified three members of LINE RT elements in the olive flounder genome. They showed high amino acid sequence identity (89–99 %), and the sequences of LINE reverse transcriptase (RT) in olive flounder are closely related to those of coral grouper, European seabass, and three-spined stickleback. Real-time reverse transcription-polymerase chain reaction analysis indicated that expression of the OF (Olive flounder)-LINE Chr3-1 RT increased more in spleen than in other tissues after treatment with the pathogen Streptococcus parauberis. These data may form the basis for further studies on the function of retroelements in infected olive flounder.

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Acknowledgments

This research was a part of the project titled “Omics based on fishery disease control technology development and industrialization (20150242),” funded by the Ministry of Oceans and Fisheries, Korea.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 46241, Korea

    Jeong-An Gim, Gyu-Hwi Nam, Hee-Eun Lee & Heui-Soo Kim

  2. Genetic Engineering Institute, Pusan National University, Busan, 46241, Korea

    Jeong-An Gim, Gyu-Hwi Nam, Hee-Eun Lee, Anshuman Mishra & Heui-Soo Kim

  3. Biotechnology Research Division, NFRDI, Busan, 46083, Korea

    Woo-Jin Kim

  4. Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, 46241, Korea

    Yunjeong Choe

  5. Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University, Tongyeong, 53064, Korea

    Chan-Il Park

  6. Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, Korea

    Do-Hyung Kim

  7. Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan, 47227, Korea

    Yung Hyun Choi

  8. Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, 49267, Korea

    Hee-Jae Cha

  9. Department of Chemistry, College of Natural Sciences, Pusan National University, Busan, 46241, Korea

    Dahye Yoon & Suhkmann Kim

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  1. Jeong-An Gim
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  13. Heui-Soo Kim
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Corresponding author

Correspondence to Heui-Soo Kim.

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Pusan National University guidelines for the care and use of animals were followed.

Conflict of interest

J-AG declares that he has no conflict of interest. G-HN declares that he has no conflict of interest. W-JK declares that he has no conflict of interest. H-EL declares that she has no conflict of interest. AM declares that he has no conflict of interest. YC declares that she has no conflict of interest. C-IP declares that he has no conflict of interest. D-HK declares that he has no conflict of interest. YHC declares that he has no conflict of interest. H-JC declares that he has no conflict of interest. DY declares that she has no conflict of interest. SK declares that he has no conflict of interest. H-SK declares that he has no conflict of interest.

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Gim, JA., Nam, GH., Kim, WJ. et al. Detection of LINE RT elements in the olive flounder (Paralichthys olivaceus) genome and expression analysis after infection with S. parauberis . Genes Genom 38, 1105–1110 (2016). https://doi.org/10.1007/s13258-016-0457-8

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  • DOI: https://doi.org/10.1007/s13258-016-0457-8

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