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Characterization of genes coding for galacturonosyltransferase-like (GATL) proteins in rice

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Abstract

In the present study, seven galacturonosyltransferase-like (GATL) genes (OsGATLs) in rice (Oryza sativa L.) were genome-widely identified and the chromosomal locations and the gene structures of which were characterized. Under normal condition, OsGATL2 and OsGATL3 are highly expressed in root, while OsGATL4 is highly expressed in stem and leaf. Many cis-elements related to stress response and plant hormone were found in the promoter sequence of each OsGATL. The expression patterns of these OsGATL genes under treatment with abscisic acid (ABA), drought and low temperature were assessed by qRT-PCR. The expression levels of most OsGATLs significantly increased following the treatments with drought or low temperature. In addition, physicochemical properties of OsGATLs and phylogenetic analysis with GATL from rice and several other species were performed. 3D structures and protein–protein interaction (PPI) network of OsGATLs were further predicted by Swiss-model and STRING 9.0 database, respectively. The identification and bioinformatic analysis of GATL family in rice could provide reference data for further study on their biological functions, especially in the responsiveness to hormones and stress signaling.

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Acknowledgments

This work was supported by the National Natural Science foundation of China (No. 31260313 and 31560558), China Postdoctoral Science Foundation (No. 2014M561880), Jiangxi postdoctoral scientific research projects according to qualification (No. 2014KY38).

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031, China

    Jinlong Liu, Mansi Luo, Xin Yan, Chao Yu & Shaobo Li

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  1. Jinlong Liu
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  2. Mansi Luo
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Correspondence to Shaobo Li.

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Liu, J., Luo, M., Yan, X. et al. Characterization of genes coding for galacturonosyltransferase-like (GATL) proteins in rice. Genes Genom 38, 917–929 (2016). https://doi.org/10.1007/s13258-016-0436-0

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