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Transcriptome analysis of Arabidopsis thaliana in response to cement dust

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Abstract

Cement dust is a major particulate air pollutants and can cause negative impacts on plant growth and development. The molecular bases of plant responses to cement dust are not elucidated. Therefore, a transcriptome analysis of Arabidopsis in response to cement dust was performed. In the present study, seedlings of Arabidopsis thaliana were exposed to cement dust at a rate of 1.5 g per 1 m2 area. Total RNA from control and cement-dust treated plants were used for transcriptome analysis using GeneChip® Arabidopsis ATH1 Genome Array. In response to cement dust application, the transcriptional profiling identified 1599 differentially expressed genes (DEGs) using a two-fold cutoff. Of these DEGs, 831 were up-regulated, and 768 were down-regulated. Differential expression of 15 genes from this list was validated using qRT-PCR. Gene ontology analysis using AgriGo program revealed that a large proportion of up-regulated DEGs are related to response to stimulus, response to stress, response to chemical stimulus, transcription factor activity, hydrolase activity, and carboxylesterase activity. While, a large proportion of down-regulated DEGs were mainly in the following categories: biosynthetic process, biological regulation, and response to stimulus, nucleic acid binding, transcription regulator activity, transcription factor activity, organelle lumen, nuclear lumen, and nucleolus. The DEGs up-regulated in response to cement dust include a set of reactive oxygen species scavenging enzymes (e.g., ascorbate peroxidase 2, peroxidases, glutathione S-transferases), heat shock proteins, late embryogenesis abundant proteins, ∆1-pyrroline-5-carboxylate synthase 1, as well as transcription factor genes of different families. This study is the first to provide a global view of the transcriptomic profiling of Arabidopsis in response to cement dust. The results will be helpful for better understanding the molecular basis for plant responses to cement dust pollution.

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Acknowledgments

This work was supported by The Scientific Research Fund, Ministry of Higher Education and Scientific Research (Jordan), Grant number Z,B/1/08/2010. We are grateful to Bayan Al-Momany, Aya Awad, Noor Atiyat and to Arefah Nuaimat for excellent technical assistance.

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  1. Jarrah Alzubi

    Present address: Arab Countries Water Utilities Association (ACWUA), Amman, 11196, Jordan

Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa’ Applied University, Al-Salt, 19117, Jordan

    Saeid Abu-Romman

  2. Department of Water Resources and Environmental Management, Faculty of Agricultural Technology, Al-Balqa’ Applied University, Al-Salt, 19117, Jordan

    Jarrah Alzubi

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  1. Saeid Abu-Romman
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Correspondence to Saeid Abu-Romman.

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Abu-Romman, S., Alzubi, J. Transcriptome analysis of Arabidopsis thaliana in response to cement dust. Genes Genom 38, 865–878 (2016). https://doi.org/10.1007/s13258-016-0432-4

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